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MABF770 | Anti-CDK9, clone 1B5D10 Antibody

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC, ELISA M Ascites Monoclonal Antibody
      Catalogue NumberMABF770
      DescriptionAnti-CDK9, clone 1B5D10 Antibody
      Alternate Names
      • Cyclin-dependent kinase 9
      • CDK9
      Background InformationCDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
      Product Information
      PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
      ApplicationDetect CDK9 using this Anti-CDK9, clone 1B5D10 Antibody validated for use in Western Blotting, Immunohistochemistry and ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected CDK9 in endometrial cancer and rectum cancer tissues.

      ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

      Biological Information
      ImmunogenPurified recombinant fragment of human CDK9 expressed in E. Coli. 
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • CDK9
      • CDC2L4
      • TAK
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat, A431 and HEK293 cell lysates.

      Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected CDK9 in Jurkat, A431 and HEK293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies