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MABF610 | Anti-CD45RA Antibody (human), PerCP-Cy5.5, clone HI100

25 tests  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, IHC, WB M PerCP-Cy5.5 Monoclonal Antibody
      Catalogue NumberMABF610
      DescriptionAnti-CD45RA Antibody (human), PerCP-Cy5.5, clone HI100
      Alternate Names
      • Receptor-type tyrosine-protein phosphatase C
      • Leukocyte common antigen
      • L-CA
      • T200
      • CD antigen CD45
      Background InformationThe HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.The HI100 antibody is widely used as a marker for human CD45RA expression on naïve and activated T cells, B cells, and monocytes.
      Product Information
      PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
      ApplicationThis mouse monoclonal antibody Anti-CD45RA (human), PerCP-Cy5.5, clone HI100 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD45RA.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Western Blotting
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      Concentration5 µL (0.06 µg)/test
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PTPRC
      • CD45
      Purification MethodSize Exclusion Chromatography
      UniProt Number
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.06 µg of this antibody detected CD45RA in one million human peripheral blood lymphocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size25 tests
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies