A part of MilliporeSigma

MABF321 | Anti-CD45 (mouse) Antibody, PE, clone 30-F11

200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC, IHC, ACT, IP, WB R PE Monoclonal Antibody
      Catalogue NumberMABF321
      DescriptionAnti-CD45 (mouse) Antibody, PE, clone 30-F11
      Alternate Names
      • Receptor-type tyrosine-protein phosphatase C
      • Leukocyte common antigen
      • L-CA
      • T200
      • CD45
      Background InformationCD45, also known as PTPRC, is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells. CD 45 belongs to the protein-tyrosine phosphatase family, receptor class 1/6 subfamily.It also contains 2 fibronectin type-III domains and 2 tyrosine-protein phosphatase domains. CD45 has been shown to play an important role in T-cell activation through the antigen receptor. CD45 also is suggested to act as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one is suggested to affect the substrate specificity of the first domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN. Furthermore, it has been shown to dephosphorylate LYN, and modulate LYN activity. Interestingly, CD45 has been associated with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. CD45 has been shown to bind with GANAB and PRKCSH. Additionally, it interacts with SKAP1 and DPP4, whereas the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Mutations in CD45 have been associated with Multiple Sclerosis (MS) and severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
      Product Information
      PresentationPurified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.
      ApplicationAnti-CD45 (mouse) Antibody, PE, clone 30-F11 is a highly specific rat monoclonal antibody, that targets CD45 & has been tested in Flow Cytometry, IHC, Activity Assay, IP & western blotting.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Activity Assay
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected human CD45 in Western Blotting. (Tsuboi S and Fukuda M. 1998)

      Immunohistochemistry Analysis: A representative lot detected human CD45 in Immunohistochemistry. (Simon DI, et al. 2000; Cornet A, et al. 2001)

      Immunoprecipitation Analysis: A representative lot detected human CD45 in Immunoprecipitation. (Ledbetter JA, et al. 1979)
      Biological Information
      ImmunogenMouse thymus or spleen
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PTPRC
      • CD45
      Purification MethodAffinity
      UniProt Number
      Molecular Weight145 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

      Flow Cytometry Analysis: 0.25 μg of this antibody detected CD45 in one million C57BL/6 mouse splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CD45 (mouse), PE, clone 30-F11 - QVP1308168 QVP1308168
      Anti-CD45 (mouse), PE, clone 30-F11 -VP1603150 VP1603150


      Reference overviewPub Med ID
      Enterocolitis induced by autoimmune targeting of enteric glial cells: a possible mechanism in Crohn's disease?
      Cornet, A, et al.
      Proc. Natl. Acad. Sci. U.S.A., 98: 13306-11 (2001) 2001

      Show Abstract
      11687633 11687633
      Decreased neointimal formation in Mac-1(-/-) mice reveals a role for inflammation in vascular repair after angioplasty.
      Simon, D I, et al.
      J. Clin. Invest., 105: 293-300 (2000) 2000

      Show Abstract
      10675355 10675355
      Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs humoral immune responses in transgenic mice.
      Tsuboi, S and Fukuda, M
      J. Biol. Chem., 273: 30680-7 (1998) 1998

      Show Abstract
      9804842 9804842
      Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.
      Ledbetter, J A and Herzenberg, L A
      Immunol. Rev., 47: 63-90 (1979) 1979

      398327 398327


      New Products Volume 5, 2013 - Antibodies, Assays, Small Modecules, Inhibitors, and Proteins