217594 | Anti-CD44 Rat mAb (A020)

217594
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, Ca, H, M, Porcine, Rb R Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      217594-100UL
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.
          Catalogue Number217594
          Brand Family Calbiochem®
          References
          ReferencesIida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
          Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesNeutralization Studies Bourguignon, L.Y.W., et al. 2007. J. Biol. Chem. 282, 1265. Bourguignon, L.Y.W., et al. 2006. J. Biol. Chem. 281 14026.
          Key Applications Immunofluorescence
          Immunohistochemistry
          Immunoprecipitation
          Radioimmunoassay
          Enzyme-Linked Immunosorbent Assay
          Flow Cytometry
          Immunoblotting (Western Blotting)
          Application NotesELISA (1:2000)
          Flow Cytometry (1:1000)
          Immunoblotting (1:1000)
          Immunofluorescence (1:200)
          Immunohistochemistry (1:250)
          Immunoprecipitation (1:200)
          Neutralization Studies (see comments and application references)
          Radioimmunoassay (1:5000)
          Application CommentsThe antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenpurified human lymphocyte CD44
          ImmunogenHuman
          CloneA020
          HostRat
          IsotypeIgG2b
          Species Reactivity
          • Bovine
          • Canine
          • Human
          • Mouse
          • Porcine
          • Rabbit
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          217594

          References

          Reference overview
          Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
          Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Biologics 32.4 ( 1.19 MB )
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-July-2007 RFH
          ApplicationELISA (1:2000)
          Flow Cytometry (1:1000)
          Immunoblotting (1:1000)
          Immunofluorescence (1:200)
          Immunohistochemistry (1:250)
          Immunoprecipitation (1:200)
          Neutralization Studies (see comments and application references)
          Radioimmunoassay (1:5000)
          DescriptionRat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
          HostRat
          Immunogen speciesHuman
          Immunogenpurified human lymphocyte CD44
          CloneA020
          IsotypeIgG2b
          Speciesbovine, canine, human, mouse, porcine, rabbit
          FormLiquid
          FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThe antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesIida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
          Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.
          Application referencesNeutralization Studies Bourguignon, L.Y.W., et al. 2007. J. Biol. Chem. 282, 1265. Bourguignon, L.Y.W., et al. 2006. J. Biol. Chem. 281 14026.