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MABF116 | Anti-CD3 gamma delta (HMT3-2) Antibody, clone Ham25-1157

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M FC Ht Purified Monoclonal Antibody
      Catalogue NumberMABF116
      DescriptionAnti-CD3 gamma delta (HMT3-2) Antibody, clone Ham25-1157
      Alternate Names
      • T-cell surface glycoprotein CD3 gamma chain
      • T-cell receptor T3 gamma chain
      • CD3g
      • HMT3-2
      Background InformationThe CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains: gamma, delta, epsilon and zeta. CD3 is first detectable in early thymocytes and one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocytes, it is detected on the cell surface. The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies.
      Product Information
      • Jurkat cells
      PresentationPurified armenian hamster monoclonal IgG2bλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-CD3 gamma delta (HMT3-2) Antibody, clone Ham25-1157 detects level of CD3 gamma delta (HMT3-2) & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenPurified protein corresponding to mouse CD3 gamma delta.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes CD3 gamma delta.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cd3g
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: The CD3 complex mediates signal transduction.

      SUBUNIT STRUCTURE: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      Molecular Weight21 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in Jurkat cells.

      Flow Cytometry Analysis: 2.5 µg/mL of this antibody detected CD3 gamma delta in 1X10E5 Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information