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MABF318 | Anti-CD3ε (mouse) Antibody, PE/Cy5, clone 145-2C11

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC, IHC, ACT, IP, WB AHm PE-Cy5 Monoclonal Antibody
      Catalogue NumberMABF318
      DescriptionAnti-CD3ε (mouse) Antibody, PE/Cy5, clone 145-2C11
      Alternate Names
      • T-cell surface glycoprotein CD3 epsilon chain
      • T-cell surface antigen T3/Leu-4 epsilon chain
      • CD3e
      Background InformationCD3, also known as T3, is a member of the Ig and the ITAM superfamilies. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon and zeta. CD3 is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The CD3 antigen is present in early thymocytes, and has been considered the best all-around T cell marker. The positive staining of this marker may represent a sign of early commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface. The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies. CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
      Product Information
      PresentationPurified armenian hamster conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide and 0.2% (w/v) BSA.
      ApplicationDetect CD3ε using this armenian hamster monoclonal antibody, Anti-CD3ε (mouse) Antibody, PE/Cy5, clone 145-2C11 validated for use in Flow Cytometry, IHC, Activity Assay, IP & western blotting.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Activity Assay
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected human CD3ε in Western Blotting. (Salvadori S, et al. 1994.)

      Immunohistochemistry Analysis: A representative lot detected human CD3ε in Immunohistochemistry. (Podd BS, et al. 2006; Tilley SL, et al. 2007)

      Immunofluorescent Analysis: A representative lot detected human CD3ε in Immunofuorecence. (Payer E, et al. 1991)

      Immunoprecipitation Analysis: A representative lot detected human CD3ε in Immunoprecipitation. (Leo O, et al. 1987.)

      Activity Assay Analysis: A representative lot detected human CD3ε in Activity Assay. (Leo O, et al. 1987; Kruisbeek AM, et al. 1991; Vossen ACTM, et al. 1995; Terrazas LI, et al. 2005; Wang W, et al. 2007.)
      Biological Information
      ImmunogenH-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostArmenian Hamster
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cd3e
      Purification MethodAffinity
      UniProt Number
      Molecular Weight21 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.

      Flow Cytometry Analysis: 1 μg of this antibody detected CD3ε in one million C57BL/6 mouse splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CD3 (mouse), PE/Cy5, clone 145-2C11 - QVP1308136 QVP1308136


      Reference overviewPub Med ID
      Retinoid-related orphan receptor gamma controls immunoglobulin production and Th1/Th2 cytokine balance in the adaptive immune response to allergen.
      Tilley, Stephen L, et al.
      J. Immunol., 178: 3208-18 (2007) 2007

      Show Abstract
      17312169 17312169
      Type 1 sphingosine 1-phosphate G protein-coupled receptor (S1P1) mediation of enhanced IL-4 generation by CD4 T cells from S1P1 transgenic mice.
      Wang, Wengang, et al.
      J. Immunol., 178: 4885-90 (2007) 2007

      Show Abstract
      17404269 17404269
      T cells in cryptopatch aggregates share TCR gamma variable region junctional sequences with gamma delta T cells in the small intestinal epithelium of mice.
      Podd, Bradley S, et al.
      J. Immunol., 176: 6532-42 (2006) 2006

      Show Abstract
      16709810 16709810
      Role of the programmed Death-1 pathway in the suppressive activity of alternatively activated macrophages in experimental cysticercosis.
      Terrazas, Luis I, et al.
      Int. J. Parasitol., 35: 1349-58 (2005) 2005

      Show Abstract
      16126211 16126211
      Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects.
      Vossen, A C, et al.
      Eur. J. Immunol., 25: 1492-6 (1995) 1995

      Show Abstract
      7614975 7614975
      Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.
      Salvadori, S, et al.
      J. Immunol., 153: 5176-82 (1994) 1994

      Show Abstract
      7963575 7963575
      Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
      Payer, E, et al.
      J. Immunol., 146: 2536-43 (1991) 1991

      Show Abstract
      1826696 1826696
      Identification of a monoclonal antibody specific for a murine T3 polypeptide.
      Leo, O, et al.
      Proc. Natl. Acad. Sci. U.S.A., 84: 1374-8 (1987) 1987

      Show Abstract
      2950524 2950524


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