A part of MilliporeSigma

04-1478 | Anti-CD275/ICOSL Antibody, clone HK5.3

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC R Purified Monoclonal Antibody
      Catalogue Number04-1478
      DescriptionAnti-CD275/ICOSL Antibody, clone HK5.3
      Alternate Names
      • B7 homolog 2
      • B7-like protein
      • B7-like protein Gl50
      • B7-related protein 1
      • CD275 antigen
      • inducible T-cell co-stimulator ligand
      • transmembrane protein B7-H2 ICOS ligand
      Background InformationInducible T-cell co-stimulator ligand (ICOS ligand, ICOSL or CD275) is a single-pass type I membrane protein for the T-cell-specific cell surface receptor ICOS, expressed on activated monocytes and dendritic cells. ICOSL/CD275 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and also stimulates B-cell proliferation and differentiation into plasma cells. ICOSL/CD275 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. There are 2 isoforms for ICOSL/CD275. Isoform 1 is widely expressed in tissues whereas isoform 2 is detected only in lymph nodes, leukocytes and the spleen. ICOSL/CD275 expression is further enhanced by treatment with TNF-alpha in peripheral blood B-cells and monocytes, while it is decreased in dendritic cells.
      Product Information
      • PMA-stimulated BALB/c splenocytes
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      ApplicationAnti-CD275/ICOSL Antibody, clone HK5.3 detects level of CD275/ICOSL & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenMouse ICOSL cDNA-transfected NRK cells.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes CD275/ ICOSL.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ICOSLG
      • B7-H2
      • B7H2
      • B7RP-1
      • B7RP1
      • CD275
      • GL50
      • ICOS-L
      • ICOSL
      • LICOS
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICTY: Isoform 1 highest expression in lymphoid tissues, such as spleen (mostly in the marginal zone), lymph nodes (particularly in the cortex and in both primary and secondary follicles), thymus (predominantly in the medulla) and Peyer patches (mostly in the follicles), lower levels in many non-lymphoid tissues, such as brain, heart, kidney, liver, lung, skeletal muscle and testis. Present on freshly isolated splenic B-cells, T-cells, dendritic cells and macrophages. The expression of isoform 2 is restricted to heart, spleen and kidney.

      DEVELOPMENTAL STAGE: Detected early in hemopoiesis: in the yolk sac at 11.5 and 12.5 dpc and, to a lesser extent, in the liver at 14.5 dpc.

      SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily. BTN/MOG family.

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
      Molecular Weight36 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in PMA-stimulated BALB/c splenocytes.

      Flow Cytometry Analysis: 10 µg of this antibody detected CD275/ICOSL in PMA-stimulated BALB/c splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information