217660 | Anti-CD18 Mouse mAb (IB4)

217660
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      217660-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.
          Catalogue Number217660
          Brand Family Calbiochem®
          SynonymsAnti-β2 Integrin
          References
          ReferencesBerman, M.E., et al. 1996. J. Immunol. 156, 1515.
          Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
          Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
          Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
          Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
          Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Negative controlNalm-6 cells
          Positive controlHPB-MLT cells
          Preservative0.5 mg/ml gentamicin sulfate
          Applications
          Key Applications Immunoprecipitation
          Cell Inhibition Assays
          Flow Cytometry
          Frozen Sections
          Application NotesFlow Cytometry (5-10 µg/ml)
          Frozen Sections (see comments)
          Immunoprecipitation (see comments)
          Cell Inhibition Assays (see comments)
          Application CommentsBlocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenhuman peripheral blood monocytes
          ImmunogenHuman
          CloneIB4
          HostMouse
          Purity≥95% by SDS-PAGE
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          217660

          References

          Reference overview
          Berman, M.E., et al. 1996. J. Immunol. 156, 1515.
          Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
          Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
          Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
          Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
          Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-July-2009 JSW
          SynonymsAnti-β2 Integrin
          ApplicationFlow Cytometry (5-10 µg/ml)
          Frozen Sections (see comments)
          Immunoprecipitation (see comments)
          Cell Inhibition Assays (see comments)
          DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).
          BackgroundHuman CD18 (β2 integrin) is expressed on leukocytes as part of a heterodimeric complex with either CD11a, b or c (integrins αL, αM, or αX). CD18/CD11 heterodimers function in cell adhesion in immune and inflammatory responses.
          HostMouse
          Immunogen speciesHuman
          Immunogenhuman peripheral blood monocytes
          CloneIB4
          IsotypeIgG2a
          Specieshuman
          Positive controlHPB-MLT cells
          Negative controlNalm-6 cells
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥95% by SDS-PAGE
          Preservative0.5 mg/ml gentamicin sulfate
          CommentsBlocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesBerman, M.E., et al. 1996. J. Immunol. 156, 1515.
          Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
          Mazzone, A., and Ricevuti, G. 1995. Haematologica 80, 161.
          Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
          Landis, R.C., et al. 1994. J. Cell Biol. 126, 529.
          Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 5699.