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ABN455 | Anti-C9ORF72/C9RANT (poly-GP) Antibody

ABN455
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) Rb Unpurified Polyclonal Antibody
      Description
      Catalogue NumberABN455
      DescriptionAnti-C9ORF72/C9RANT (poly-GP) Antibody
      Alternate Names
      • C9ORF72
      • C9RANT
      Background InformationIn a noncoding region of C9ORF72 hexanucleotide (GGGGCC) repeat expansions are the major genetic cause of FTD and ALS (c9FTD/ALS). Transcripts that have the RNA structure of GGGGCC repeats are susceptible to an unconventional mechanism of translation called repeatassociated non-ATG (RAN) translation. Translation of the GGGGCC-repeat in all reading frames would result in three dipeptide-repeat (DPR) proteins poly-(Gly-Ala), poly-(Gly-Pro) and poly-(Gly-Arg). poly-GA and poly-GP proteins are extremely hydrophobic and may form intracellular aggregates. Anti-C9RANT, an antibody that was generated against putative GGGGCC repeat RANtranslated peptides mentioned above, detects insoluble high molecular weight material in brain homogenates, and neuronal inclusions throughout the CNS of c9FTD/ALS cases (Ash, PE, et al. (2013). Neuron. 77(4):639-46.)
      References
      Product Information
      FormatUnpurified
      PresentationRabbit Polyclonal serum with 0.05% sodium azide.
      Applications
      ApplicationAnti-C9ORF72/C9RANT (poly-GP), Cat # ABN455, is a highly validated rabbit Polyclonal antibody, that targets C9ORF72/C9RANT (poly-GP) and has been tested in Western Blotting and Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:5000 dilution from a representative lot detected C9RANT (poly-GP) in human cerebellar sections of c9FTD cases (Ash, PE, et al. (2013). Neuron. 77(4):639-646.).
      Biological Information
      ImmunogenLinear peptides corresponding to C-Ahx-(GA)8-amide, C-Ahx-(GP)8-amide, and C-Ahx-
      (GR)8-amide
      Epitopepoly-GP
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with all species.
      Antibody TypePolyclonal Antibody
      Purification MethodUnpurified
      Molecular WeightVariable
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in transfected HEK293 cell lysate.

      Western Blotting Analysis: 1:2000 diultion of this antibody detected GST fusion protein with 5 GP repeats in 10 µg of transfected HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-C9ORF72/C9RANT (poly-GP) -2616199 2616199
      Anti-C9ORF72/C9RANT (poly-GP) -2685214 2685214
      Anti-C9ORF72/C9RANT (poly-GP) -2787654 2787654
      Anti-C9RANT - Q2468270 Q2468270

      References

      Reference overviewPub Med ID
      Unconventional translation of C9ORF72 GGGGCC expansion generates insoluble polypeptides specific to c9FTD/ALS.
      Ash, Peter E A, et al.
      Neuron, 77: 639-46 (2013) 2013

      Show Abstract
      23415312 23415312