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AB1846 | Anti-C-X-C Chemokine Receptor 4 Antibody, NT

AB1846
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC, IP, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1846
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-C-X-C Chemokine Receptor 4 Antibody, NT
      Alternate Names
      • CD184
      • CXCR4
      References
      Product Information
      FormatPurified
      Control
      • Skin
      PresentationPurified IgG in PBS containing 0.02% sodium azide.
      Applications
      ApplicationAnti-C-X-C Chemokine Receptor 4 Antibody, N-terminus is an antibody against C-X-C Chemokine Receptor 4 for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: 1-2 μg/mL. HeLa cell lysates can be used as positive control. The antibody should detect a band at 40 kDa.

      Immunocytochemistry: 10 μg/mL

      Immunoprecipitation

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenAmino-terminus peptide of human CXCR4 (Berson, 1996; Leotscher, 1994).
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes CXCR4, N-terminus.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
      Gene Symbol
      • CXCR4
      • WHIM
      • NPY3R
      • NPYY3R
      • FB22
      • LAP3
      • Fusin
      • CXCR-4
      • LCR1
      • NPYR
      • HM89
      • fusin
      • HSY3RR
      • CXC-R4
      • NPYY3
      • NPYRL
      • D2S201E
      • CD184
      • LESTR
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P61073 # Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
      SIZE: 352 amino acids; 39746 Da
      SUBUNIT: Interacts with HIV-1 surface protein gp120 and Tat.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
      DOMAIN: SwissProt: P61073 The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
      PTM: Sulfated.
      DISEASE: SwissProt: P61073 # Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also called warts, hypogammaglobulinemia, infections, and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
      SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
      Molecular Weight40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain 1 year at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      ANTI-CXCR4 -2691395 2691395
      RABBIT ANTI-CXCR4 (N-TERMINUS) 2483169
      RABBIT ANTI-CXCR4 (N-TERMINUS) -2716580 2716580
      RABBIT ANTI-CXCR4 (N-TERMINUS) POLYCLONAL ANTIBODY - 2382585 2382585
      RABBIT ANTI-CXCR4 (N-TERMINUS) POLYCLONAL ANTIBODY - 2040360 2040360
      RABBIT ANTI-CXCR4 (N-TERMINUS) POLYCLONAL ANTIBODY - 2295409 2295409
      RABBIT ANTI-CXCR4 (N-TERMINUS) POLYCLONAL ANTIBODY -2649226 2649226
      RABBIT ANTI-CXCR4 (N-TERMINUS) POLYCLONAL ANTIBODY -2760637 2760637
      RABBIT ANTI-CXCR4 (N-TERMINUS) POLYCLONAL ANTIBODY -2772782 2772782

      References | 18 Available | See All References

      Reference overviewApplicationPub Med ID
      Effects and mechanisms of blocking the hedgehog signaling pathway in human gastric cancer cells.
      Gu, H; Li, XU; Zhou, C; Wen, Y; Shen, Y; Zhou, L; Li, J
      Oncology letters 9 1997-2002 2015

      Show Abstract
      26137001 26137001
      Tyrosylprotein sulfotransferase-1 and tyrosine sulfation of chemokine receptor 4 are induced by Epstein-Barr virus encoded latent membrane protein 1 and associated with the metastatic potential of human nasopharyngeal carcinoma.
      Xu, J; Deng, X; Tang, M; Li, L; Xiao, L; Yang, L; Zhong, J; Bode, AM; Dong, Z; Tao, Y; Cao, Y
      PloS one 8 e56114 2013

      Show Abstract
      Western Blotting23472069 23472069
      Androgens Induce Functional CXCR4 through ERG Factor Expression in TMPRSS2-ERG Fusion-Positive Prostate Cancer Cells.
      Cai, Juan, et al.
      Transl Oncol, 3: 195-203 (2010) 2010

      Show Abstract
      20563261 20563261
      Nitric oxide donor upregulation of stromal cell-derived factor-1/chemokine (CXC motif) receptor 4 enhances bone marrow stromal cell migration into ischemic brain after stroke.
      Cui, X; Chen, J; Zacharek, A; Li, Y; Roberts, C; Kapke, A; Savant-Bhonsale, S; Chopp, M
      Stem cells (Dayton, Ohio) 25 2777-85 2007

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)17641243 17641243
      Differential functional activation of chemokine receptor CXCR4 is mediated by G proteins in breast cancer cells
      Holland, Jane D, et al
      Cancer Res, 66:4117-24 (2006) 2006

      16618732 16618732
      Stromal cell-derived factor 1alpha mediates neural progenitor cell motility after focal cerebral ischemia.
      Robin, AM; Zhang, ZG; Wang, L; Zhang, RL; Katakowski, M; Zhang, L; Wang, Y; Zhang, C; Chopp, M
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 26 125-34 2006

      Show Abstract
      15959456 15959456
      Transactivation of CXCR4 by the insulin-like growth factor-1 receptor (IGF-1R) in human MDA-MB-231 breast cancer epithelial cells
      Akekawatchai, Chareeporn, et al
      J Biol Chem, 280:39701-8 (2005) 2005

      16172123 16172123
      CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow.
      Avigdor, A; Goichberg, P; Shivtiel, S; Dar, A; Peled, A; Samira, S; Kollet, O; Hershkoviz, R; Alon, R; Hardan, I; Ben-Hur, H; Naor, D; Nagler, A; Lapidot, T
      Blood 103 2981-9 2004

      Show Abstract
      15070674 15070674
      Role of the first and third extracellular domains of CXCR-4 in human immunodeficiency virus coreceptor activity.
      Brelot, A, et al.
      J. Virol., 71: 4744-51 (1997) 1997

      Show Abstract
      9151868 9151868
      Evolution of HIV-1 coreceptor usage through interactions with distinct CCR5 and CXCR4 domains.
      Lu, Z, et al.
      Proc. Natl. Acad. Sci. U.S.A., 94: 6426-31 (1997) 1997

      Show Abstract
      9177234 9177234

      Data Sheet

      Title
      Anti-C-X-C Chemokine Receptor 4, N-terminus - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies