A part of MilliporeSigma

MAB3612 | Anti-BubR1 Antibody, clone 8G1

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMAB3612
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BubR1 Antibody, clone 8G1
      Background InformationThe mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. BubR1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.
      Product Information
      ApplicationDetect BubR1 with Anti-BubR1 Antibody, clone 8G1 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern blot using an ECL detection system. Reacts with the ~120 kDa BubR1 protein and higher bands due to hyperphosphorylations.

      Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant human BubR1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes human BubR1.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
      Gene Symbol
      • BUB1B
      • Bub1A
      • hBUBR1
      • BUB1beta
      • SSK1
      • BUBR1
      • MAD3L
      • EC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O60566 # Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
      SIZE: 1050 amino acids; 119545 Da
      SUBUNIT: Interacts with CENPE, CENPF, mitosin and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase- promoting complex/cyclosome (APC/C).
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Kinetochore. Note=Cytoplasmic in interphase cells. Associates with the kinetochores.
      TISSUE SPECIFICITY: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
      DOMAIN: SwissProt: O60566 The CD1 domain directs kinetochore localization and binding to BUB3. & The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase (Potential).
      PTM: Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp- 610. & Ubiquitinated (Probable). Degradated by the proteasome. & Autophosphorylated in vitro. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells.
      DISEASE: SwissProt: O60566 # Defects in BUB1B are associated with tumor formation. & Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant. & Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene.
      SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. & Contains 1 CD1 domain. & Contains 1 protein kinase domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-BUBR1 -2528724 2528724
      MOUSE ANTI-BUBR1 -2785819 2785819


      Reference overviewPub Med ID
      MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C.
      Voets, E; Wolthuis, R
      Biology open 4 484-95 2015

      Show Abstract
      25750436 25750436
      Formation of stable attachments between kinetochores and microtubules depends on the B56-PP2A phosphatase.
      Foley, EA; Maldonado, M; Kapoor, TM
      Nature cell biology 13 1265-71 2011

      Show Abstract
      21874008 21874008
      Overexpression of the dynein light chain km23-1 in human ovarian carcinoma cells inhibits tumor formation in vivo and causes mitotic delay at prometaphase/metaphase.
      Pulipati, Nageswara R, et al.
      Int. J. Cancer, 129: 553-64 (2011) 2011

      Show Abstract
      21469138 21469138
      Heat shock protein inhibitors, 17-DMAG and KNK437, enhance arsenic trioxide-induced mitotic apoptosis.
      Yi-Chen Wu,Wen-Yen Yen,Te-Chang Lee,Ling-Huei Yih
      Toxicology and applied pharmacology 236 2009

      Show Abstract
      19371599 19371599
      Subcellular localization of the spindle proteins Aurora A, Mad2, and BUBR1 assessed by immunohistochemistry.
      Burum-Auensen, E; De Angelis, PM; Schjølberg, AR; Kravik, KL; Aure, M; Clausen, OP
      The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 55 477-86 2007

      Show Abstract
      17242465 17242465

      Data Sheet

      Anti-BubR1, clone 8G1 - Data Sheet

      Related Products & Applications

      Related Products

      Catalog Number Description  
      ABS158 Anti-BubR1 Antibody Show Pricing & Availability

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies