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AB5945 | Anti-Brn-3a Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, IHC, WB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB5945
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Brn-3a Antibody
      Product Information
      FormatAffinity Purified
      • Widely expressed
      PresentationAffinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      ApplicationDetect Brn-3a using this Anti-Brn-3a Antibody validated for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:200-1:1,000 using ECL.

      Immunohistochemistry: 1:200-1:1,000.

      Immunocytochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenA synthetic peptide corresponding to human Brn3a (amino acids 141-157) conjugated to BSA.
      SpecificityRecognizes Brn3a. The molecular weight of the protein is 42.9kD.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryBRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing sensory nervous system and in cells of the B- and T-lymphocytic lineages (Gerrero et al., 1993 [PubMed 8248179]).[supplied by OMIM]
      Gene Symbol
      • POU4F1
      • FLJ13449
      • Brn-3A
      • RDC-1
      • Oct-T1
      • BRN3A
      • RDC1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q01851 # Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
      SIZE: 423 amino acids; 42940 Da
      TISSUE SPECIFICITY: Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
      DEVELOPMENTAL STAGE: Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.
      SIMILARITY: SwissProt: Q01851 ## Belongs to the POU transcription factor family. Class- 4 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
      Molecular Weight42.9 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.


      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Brn3a 2465129
      Anti-Brn3a - 2358847 2358847
      Anti-Brn3a - 2430357 2430357
      Anti-Brn3a - 1997357 1997357
      Anti-Brn3a - 2069332 2069332
      Anti-Brn3a - 2519294 2519294
      Anti-Brn3a - NG1777603 NG1777603
      Anti-Brn3a - NG1873251 NG1873251
      Anti-Brn3a -2552373 2552373
      Anti-Brn3a -2578101 2578101
      Anti-Brn3a -2636717 2636717
      Anti-Brn3a -2681238 2681238
      Anti-Brn3a -2709673 2709673
      Anti-Brn3a -2746566 2746566
      Anti-Brn3a -2806192 2806192


      Reference overviewPub Med ID
      Role of Pax3 acetylation in the regulation of Hes1 and Neurog2.
      Ichi, S; Boshnjaku, V; Shen, YW; Mania-Farnell, B; Ahlgren, S; Sapru, S; Mansukhani, N; McLone, DG; Tomita, T; Mayanil, CS
      Molecular biology of the cell 22 503-12 2011

      Show Abstract Full Text Article
      21169561 21169561
      Folic acid remodels chromatin on Hes1 and Neurog2 promoters during caudal neural tube development.
      Ichi, S; Costa, FF; Bischof, JM; Nakazaki, H; Shen, YW; Boshnjaku, V; Sharma, S; Mania-Farnell, B; McLone, DG; Tomita, T; Soares, MB; Mayanil, CS
      The Journal of biological chemistry 285 36922-32 2010

      Show Abstract
      20833714 20833714
      Key basic helix-loop-helix transcription factor genes Hes1 and Ngn2 are regulated by Pax3 during mouse embryonic development.
      Hiromichi Nakazaki, Anvesh C Reddy, Barbara L Mania-Farnell, Yueh-Wei Shen, Shunsuke Ichi, Christopher McCabe, David George, David G McLone, Tadanori Tomita, C S K Mayanil
      Developmental biology 316 510-23 2008

      Show Abstract
      18308300 18308300
      Histone deacetylase inhibition-mediated differentiation of RGC-5 cells and interaction with survival.
      Schwechter, BR; Millet, LE; Levin, LA
      Investigative ophthalmology & visual science 48 2845-57 2007

      Show Abstract Full Text Article
      17525221 17525221
      Coexpression of Brn-3a POU protein with p53 in a population of neuronal progenitor cells is associated with differentiation and protection against apoptosis.
      Chantelle D Hudson, Jennifer Podesta, Deborah Henderson, D S Latchman, V Budhram-Mahadeo
      Journal of neuroscience research 78 803-14 2004

      Show Abstract
      15532030 15532030
      Expression patterns of erythropoietin and its receptor in the developing midbrain.
      W Knabe, F Knerlich, S Washausen, T Kietzmann, A L Sirén, G Brunnett, H J Kuhn, H Ehrenreich
      Anatomy and embryology 207 503-12 2004

      Show Abstract
      14770308 14770308

      Data Sheet

      Anti-Brn-3a - Data Sheet

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