A part of MilliporeSigma

MAB3510 | Anti-BrdU Antibody, clone BU-1

100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A FC, ICC, IHC M Culture Supernatant Monoclonal Antibody
      Catalogue NumberMAB3510
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BrdU Antibody, clone BU-1
      Alternate Names
      • BrdU
      Background InformationBromodeoxyuridine (BrdU) is a thymidine analog that, when added exogenously, is incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to label proliferating cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells undergoing replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously.
      Product Information
      FormatCulture Supernatant
      • Brdu treated cells
      PresentationHybridoma culture supernatant from a perfusion system, filtered through a 0.2 micron membrane prior to vialing. Product contains 20% FBS and Ciprofloxacin at a final concentration of 10 μg/mL. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/contamination.
      ApplicationUse mouse monoclonal Anti-BrdU Antibody, clone BU-1, validated in Flow Cytometry, Immunocytochemistry, and Immunohistochemistry for the detection of bromodeoxyuridine (BrdU).
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesAlthough originally reported to stain nuclear DNA BrdU incorporation in ethanol-fixed cells without acid or base denaturation, this property was later attributed to the DNase activity present in a mycoplasma-contaminated BU-1 hybridoma culture supernatant used (Gonchoroff, N.J., et al. (1986). J. Immunol. Methods. 93(1):97-101; Greipp, P.R., et al. (1985). Am. J. Hematol. 20(3):289-292). Please include an appropriate DNA denaturation step during sample preparation prior to immunostaining using this product.
      Biological Information
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityClone BU-1 specifically binds to bromodeoxyuridine with 10% crossreactivity toward iodouridine (10%). Clone BU-1 does not crossreact with fluorodeoxyuridine or any endogenous cellular components such as thymidine or uridine.
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodUnpurified
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-BrdU Antibody, clone BU-1 -2745643A 2745643A
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - 2115988 2115988
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - 2019966 2019966
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - 2187371 2187371
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - 2343124 2343124
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - LV1747805 LV1747805
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 - NG1867684 NG1867684
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2521466 2521466
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2703711 2703711
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2729779 2729779
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2745643 2745643
      Anti-Bromodeoxyuridine, no acid or proteinase required, clone BU-1 -2772671 2772671

      References | 21 Available | See All References

      Reference overviewApplicationPub Med ID
      Endogenous CNTF mediates stroke-induced adult CNS neurogenesis in mice.
      Kang, SS; Keasey, MP; Arnold, SA; Reid, R; Geralds, J; Hagg, T
      Neurobiology of disease 49 68-78 2013

      Show Abstract
      22960105 22960105
      Lens regeneration in axolotl: new evidence of developmental plasticity.
      Suetsugu-Maki, R; Maki, N; Nakamura, K; Sumanas, S; Zhu, J; Del Rio-Tsonis, K; Tsonis, PA
      BMC biology 10 103 2012

      Show Abstract
      23244204 23244204
      What role do annelid neoblasts play? A comparison of the regeneration patterns in a neoblast-bearing and a neoblast-lacking enchytraeid oligochaete.
      Myohara, M
      PloS one 7 e37319 2012

      Show Abstract
      22615975 22615975
      Serotonin 1A receptor agonist increases species- and region-selective adult CNS proliferation, but not through CNTF.
      Arnold, SA; Hagg, T
      Neuropharmacology 63 1238-47 2012

      Show Abstract
      22884499 22884499
      D-Serine regulates proliferation and neuronal differentiation of neural stem cells from postnatal mouse forebrain.
      Xu Huang,Hui Kong,Mi Tang,Ming Lu,Jian-Hua Ding,Gang Hu
      CNS neuroscience & therapeutics 18 2012

      Show Abstract
      22280157 22280157
      Foxg1 has an essential role in postnatal development of the dentate gyrus.
      Tian, C; Gong, Y; Yang, Y; Shen, W; Wang, K; Liu, J; Xu, B; Zhao, J; Zhao, C
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 2931-49 2012

      Show Abstract
      22378868 22378868
      The bone morphogenetic protein antagonist noggin protects white matter after perinatal hypoxia-ischemia.
      Dizon ML, Maa T, Kessler JA
      Neurobiol Dis 2011

      Show Abstract
      21310236 21310236
      A complement receptor c5a antagonist regulates epithelial to Mesenchymal transition and crystallin expression after lens cataract surgery in Mice.
      Suetsugu-Maki R, Maki N, Fox TP, Nakamura K, Cowper Solari R, Tomlinson CR, Qu H, Lambris JD, Tsonis PA
      Molecular vision 17 949-64. 2011

      Full Text Article
      21541266 21541266
      Changes in global histone modifications during dedifferentiation in newt lens regeneration.
      Maki, N; Tsonis, PA; Agata, K
      Molecular vision 16 1893-7 2010

      Show Abstract
      Immunohistochemistry21031136 21031136
      Hypoxia-ischemia induces an endogenous reparative response by local neural progenitors in the postnatal mouse telencephalon.
      Dizon, M; Szele, F; Kessler, JA
      Developmental neuroscience 32 173-83 2010

      Show Abstract Full Text Article
      20616554 20616554

      Related Products & Applications

      Alternative Format

      Catalog Number Description  
      MAB3510P Anti-BrdU Antibody, clone BU-1, Peroxidase conjugated Show Pricing & Availability

      Product Families

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies