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05-1322 | Anti-Bmi-1 Antibody, clone DC9

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IP M Ascites Monoclonal Antibody
      Catalogue Number05-1322
      DescriptionAnti-Bmi-1 Antibody, clone DC9
      Alternate Names
      • B lymphoma Mo-MLV insertion region 1 homolog
      • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
      • BMI1 polycomb ring finger oncogene
      • Polycomb group RING finger protein 4
      • RING finger protein 51
      • murine leukemia viral (bmi-1) oncogene homolog
      • oncogene BMI-1
      • polycomb group ring finger 4
      Background InformationThe Bmi-1 protein is a member of the polycomb group (PcG) proteins. Bmi-1 has been reported as an oncogene by regulating the cell cycle inhibitors p16 and p19. Bmi-1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi-1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi-1 may regulate the self-renewal of other types of somatic stem cells, and it is also thought to inhibit aging in neurons through the suppression of p53. Several lines of evidence implicate Bmi-1 in tumorigenesis. Differential expression of Bmi-1 in a mouse model of metastatic prostate cancer vs. wild-type suggests Bmi-1 expression levels may predict response to therapy and survival in multiple types of cancer.
      Product Information
      • U20S Cell Lysate
      PresentationMouse monoclonal IgG1κ ascites in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
      ApplicationUse Anti-Bmi-1 Antibody, clone DC9 (Mouse Monoclonal Antibody) validated in WB, IP to detect Bmi-1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation:
      This antibody has been demonstrated by an outside laboratory to be suitable for immunoprecipitation1.
      Biological Information
      ImmunogenRecombinant fusion protein of full length murine BMI-1 tagged with MBP.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes murine BMI-1 (~35 kDa).
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Not tested in other species.

      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MGC12685
      • PCGF4
      • RNF51
      • flvi-2/bmi-1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Ref.8 Ref.11

      SUBUNIT STRUCTURE: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Ref.11 Ref.4 Ref.5 Ref.7 Ref.9

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Ref.11

      PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.

      DISEASE: Cooperates with the MYC oncogene to produce B-lymphomas.

      SIMILARITY: Contains 1 RING-type zinc finger.

      Molecular Weightapprox. 35 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on U20S cell lysate.

      Western Blot Analysis:
      1:5,000 of this lot detected BMI-1 on 15 μg/mL of U20S cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Bmi-1, clone DC9 - 2392286 2392286
      Anti-Bmi-1, clone DC9 - 2199632 2199632
      Anti-Bmi-1, clone DC9 - 2314804 2314804
      Anti-Bmi-1, clone DC9 - NG1585091 NG1585091
      Anti-Bmi-1, clone DC9 - NG1923488 NG1923488
      Anti-Bmi-1, clone DC9 -2508915 2508915


      Reference overviewApplicationPub Med ID
      Phospho-ΔNp63α/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas.
      Ratovitski, EA
      Cell cycle (Georgetown, Tex.) 13 749-61 2014

      Show Abstract
      Western Blotting, Immunoprecipitation24394434 24394434
      Biphasic euchromatin-to-heterochromatin transition on the KSHV genome following de novo infection.
      Toth, Z; Brulois, K; Lee, HR; Izumiya, Y; Tepper, C; Kung, HJ; Jung, JU
      PLoS pathogens 9 e1003813 2013

      Show Abstract
      24367262 24367262
      Targeting of the Bmi-1 oncogene/stem cell renewal factor by microRNA-128 inhibits glioma proliferation and self-renewal.
      Godlewski, Jakub, et al.
      Cancer Res., 68: 9125-30 (2008) 2008

      19010882 19010882
      Correlation of Bmi-1 expression and telomerase activity in human ovarian cancer.
      Zhang, F B, et al.
      Br. J. Biomed. Sci., 65: 172-7 (2008) 2008

      19181034 19181034
      Low BMI-1 expression is associated with an activated BMI-1-driven signature, vascular invasion, and hormone receptor loss in endometrial carcinoma.
      Engelsen, I B, et al.
      Br. J. Cancer, 98: 1662-9 (2008) 2008

      18475299 18475299
      Association between BMI-1 expression, acute graft-versus-host disease, and outcome following allogeneic stem cell transplantation from HLA-identical siblings in chronic myeloid leukemia.
      Mohty, Mohamad, et al.
      Blood, 112: 2163-6 (2008) 2008

      Show Abstract
      18565849 18565849

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