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MAB17001-50UG | Anti-Bim Antibody, clone 14A8

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M FC, IHC, IP, WB R Purified Monoclonal Antibody
      Catalogue NumberMAB17001-50UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Bim Antibody, clone 14A8
      OverviewPurity: The antibody is shown to be >95% pure as determined by SDS-PAGE. The BimS antibody is supplied 0.22 micron filtered.
      Background InformationBim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.
      Product Information
      • Skin/Skin Tumor
      PresentationProtein A Purified mouse immunoglobulin in PBS containing 0.05% Bronidox as preservative.
      ApplicationAnti-Bim Antibody, clone 14A8 is an antibody against Bim for use in FC, IH, IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesThe antibody is intended for the detection of all splice variants of Bim in a range of techniques including:

      Western blot: ~17-23kDa on reduced westerns; Bim(L) ~19kDa; ~23 kDa BimEL, Bim(s) ~17kDa (very difficult to detect at normal levels) ; endogenous levels are difficult to detect without prior immunoprecipitation of Bim isoforms in most cases. See O'Reilly, 2000 for complete details.




      It should be noted that the levels of antibody quoted here are intended to serve as a guide only. The optimal concentration should be determined empirically by the end user.
      Biological Information
      ImmunogenRecombinant mouse BimShort (BimS) protein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityPurified rat monoclonal antibody IgG2a Kappa isotype raised against recombinant mouse BimShort (BimS) protein. The antibody recognises a reactive epitope that is common to all splice variants of Bim including BimExtra Long, BimLong and BimShort. These antibodies can be used for the detection of Bim by standard techniques including Western blot, immunoprecipitation, immunohistochemistry and FACS. This antibody is highly sensitive and can, in most cases, recognize endogenous levels of BimS*.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFunction: Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
      Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity.
      Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity.
      Isoform BimEL: Mitochondrion
      Isoform BimL: Mitochondrion
      Isoform BimS: Mitochondrion
      Isoform Bim-alpha1: Mitochondrion
      Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3
      Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity.
      Sequence similarities: Belongs to the Bcl-2 family.
      Molecular Weight~17-23kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain 6 months at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      ANTI-BIM MONOCLONAL ANTIBODY - 2391097 2391097
      ANTI-BIM MONOCLONAL ANTIBODY - 2427490 2427490
      ANTI-BIM MONOCLONAL ANTIBODY - 2283842 2283842
      Anti-BIM Monoclonal Antibody -


      Reference overviewPub Med ID
      The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells
      O'Reilly, L A, et al
      Am J Pathol, 157:449-61 (2000) 2000

      10934149 10934149
      The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex.
      Puthalakath, H, et al.
      Mol. Cell, 3: 287-96 (1999) 1999

      10198631 10198631
      Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins.
      O'Reilly, L A, et al.
      BioTechniques, 25: 824-30 (1998) 1998

      9821584 9821584


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      Alternative Packsize

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      MAB17001-K Anti-Bim, Clone 14A8 Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Primary Antibodies