OP91 Anti-Bcl-2 (Ab-3) Mouse mAb (4D7)

OP91
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      OP91-100UG
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          Plastic ampoule 100 μg
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          Description
          Overview

          This product has been discontinued.



          Recognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, breast carcinoma, and lymph node tissue.

          Catalogue NumberOP91
          Brand Family Calbiochem®
          References
          ReferencesLarsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
          Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
          Silverman, G.A., et al. 1993 Mol. Cell. Biol. 13, 5469.
          Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
          Vaux, D.L., 1993. Proc. Natl. Acad. Sci. USA 90, 786.
          Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
          Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
          Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
          Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative controlBrain tissue
          Positive controlMCF-7 cells or breast carcinoma or lymph node tissue
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoprecipitation Tanaka, S., et al. 1993. J. Biol. Chem. 268, 10920. Immunofluorescence, Flow Cytometry Aiello, O., et al. 1992. Cytometry 13, 502. Immunoblotting Reed, J.C., et al. 1990. Anal. Biochem. 205, 70. Flow Cytometry Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.
          Key Applications Immunoprecipitation
          Paraffin Sections
          Flow Cytometry
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesFlow Cytometry (see application references)
          Immunoblotting (2 µg/ml, see application references)
          Immunofluorescence (see application references)
          Immunoprecipitation (see application references)
          Paraffin Sections (1 µg/ml)
          Application CommentsOnly random cells will stain for bcl-2 in brain tissue. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (ASRDPVARTSPLQTPA) corresponding to amino acids 61-76 of human Bcl-2
          ImmunogenHuman
          Clone4D7
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Bcl-2 (Ab-3) Mouse mAb (4D7) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Bcl-2 (Ab-3) Mouse mAb (4D7) Certificates of Analysis

          TitleLot Number
          OP91

          References

          Reference overview
          Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
          Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
          Silverman, G.A., et al. 1993 Mol. Cell. Biol. 13, 5469.
          Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
          Vaux, D.L., 1993. Proc. Natl. Acad. Sci. USA 90, 786.
          Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
          Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
          Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
          Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.

          Citations

          Title
        • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-October-2007 RFH
          ApplicationFlow Cytometry (see application references)
          Immunoblotting (2 µg/ml, see application references)
          Immunofluorescence (see application references)
          Immunoprecipitation (see application references)
          Paraffin Sections (1 µg/ml)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse SP2/0 cells. Recognizes the ~24 kDa Bcl-2 protein.
          BackgroundThe bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a ~24-26 kDa protein which has been localized to the inner mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death." It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock and irradiation.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic peptide (ASRDPVARTSPLQTPA) corresponding to amino acids 61-76 of human Bcl-2
          Clone4D7
          IsotypeIgG₁
          Specieshuman, not mouse, not rat
          Positive controlMCF-7 cells or breast carcinoma or lymph node tissue
          Negative controlBrain tissue
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsOnly random cells will stain for bcl-2 in brain tissue. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesLarsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
          Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
          Silverman, G.A., et al. 1993 Mol. Cell. Biol. 13, 5469.
          Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
          Vaux, D.L., 1993. Proc. Natl. Acad. Sci. USA 90, 786.
          Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
          Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
          Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
          Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
          Citation
        • Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
        • Application referencesImmunoprecipitation Tanaka, S., et al. 1993. J. Biol. Chem. 268, 10920. Immunofluorescence, Flow Cytometry Aiello, O., et al. 1992. Cytometry 13, 502. Immunoblotting Reed, J.C., et al. 1990. Anal. Biochem. 205, 70. Flow Cytometry Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.