PC68 Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb

PC68
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, Op Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC68-100UG
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, intestine, and lymph node tissue.
          Catalogue NumberPC68
          Brand Family Calbiochem®
          References
          ReferencesKozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
          Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
          Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
          Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
          Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
          Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
          Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
          Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
          Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Negative controlBrain tissue
          Positive controlHuman intestine or lymph node tissue or purified Bcl-2 (Cat. No. PF126)
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesFrozen sections (5 µg/ml)
          Immunoblotting (5 µg/ml)
          Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
          Application CommentsBrain tissue may be used as a negative control, as only random cells stain positively. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (HYKLSQRGYEWDAGD) corresponding to amino acids 20-34 of human Bcl-2
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Opossum
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Bcl-2 (Ab-2) (20-34) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC68

          References

          Reference overview
          Kozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
          Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
          Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
          Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
          Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
          Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
          Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
          Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
          Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.

          Citations

          Title
        • Denis Grandgirard, et al. (1998) Alphaviruses induce apoptosis in Bcl-2-overexpressing cells: evidence for a caspase-mediated, proteolytic inactivation of Bcl-2. The EMBO Journal 17, (1268- 1278).
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-February-2008 JSW
          ApplicationFrozen sections (5 µg/ml)
          Immunoblotting (5 µg/ml)
          Paraffin sections (2.5 µg/ml, pressure cooker pre-treatment required)
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~24-26 kDa Bcl-2 protein.
          BackgroundThe bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a 24-26 kDa protein which has been localized to the inner mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death." It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock, and irradiation.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (HYKLSQRGYEWDAGD) corresponding to amino acids 20-34 of human Bcl-2
          IsotypeIgG
          Specieshuman, mouse, opossum
          Positive controlHuman intestine or lymph node tissue or purified Bcl-2 (Cat. No. PF126)
          Negative controlBrain tissue
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsBrain tissue may be used as a negative control, as only random cells stain positively. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesKozopas, K.M. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
          Larsen, C.J., et al. 1993. Nouv. Rev. Fr. Hematol. 35, 37.
          Silverman, G.A., et al. 1993. Mol. Cell. Biol. 13, 5469.
          Vaux, D.L. 1993. Proc. Natl. Acad. Sci.USA 90, 786.
          Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
          Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
          Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
          Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370.
          Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
          Citation
        • Denis Grandgirard, et al. (1998) Alphaviruses induce apoptosis in Bcl-2-overexpressing cells: evidence for a caspase-mediated, proteolytic inactivation of Bcl-2. The EMBO Journal 17, (1268- 1278).