AM03 Anti-Bak (Ab-1) Mouse mAb (TC-100)

AM03
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      Overview

      Replacement Information

      Key Specifications Table

      HostAntibody Type
      M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      AM03-100UG
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          Plastic ampoule 100 μg
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          Description
          Overview

          This product has been discontinued.





          Recognizes the ~30 kDa (apparent MW) Bak protein in HL-60 cells.
          Catalogue NumberAM03
          Brand Family Calbiochem®
          SynonymsAnti-Bcl-2 Antagonist Killer
          Application Data
          Detection of human Bak by immunoblotting. Sample: Whole cell lysates from untreated HL-60 cells. Primary antibody: Anti-Bak (Ab-1) Mouse mAb (TC-100) (Cat. No. AM03) (2 µg/ml). Detection: chemiluminescence.
          References
          ReferencesChittenden, T., et al. 1995. Nature 374, 733.
          Farrow, S.N., et al. 1995. Nature 374, 731.
          Kiefer, M.C., et al. 1995. Nature 736.
          Reed, J.C. 1994. J. Cell Biol. 124, 1.
          Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Positive controlHL-60 cells
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunocytochemistry Panaretakis, T., et al. 2002. J. Biol. Chem. 277, 44317.
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence)
          Immunocytochemistry (see comments; see application references)
          Application CommentsThis antibody recognizes active/activated Bak under nondenaturing, native conditions using applications like immunocytochemistry (see application references). Antibody should be titrated for optimal results in individual systems for each application.
          Biological Information
          Immunogenrecombinant human Bak from which the C-terminal and transmembrane domains have been deleted
          ImmunogenHuman
          EpitopeWithin the N-terminal region
          CloneTC-100
          HostMouse
          IsotypeIgG2a
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Bak (Ab-1) Mouse mAb (TC-100) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Bak (Ab-1) Mouse mAb (TC-100) Certificates of Analysis

          TitleLot Number
          AM03

          References

          Reference overview
          Chittenden, T., et al. 1995. Nature 374, 733.
          Farrow, S.N., et al. 1995. Nature 374, 731.
          Kiefer, M.C., et al. 1995. Nature 736.
          Reed, J.C. 1994. J. Cell Biol. 124, 1.
          Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.

          Citations

          Title
        • Lena Thyrell, et al. (2004) Interferon α-induced apoptosis in tumor cells is mediated through the phosphoinositide 3-kinase/mammalian target of rapamycin signaling pathways. Journal of Biological Chemistry 279, 24152-24162.
        • Aleksandra Mandic, et al. (2001) Cisplatin induces the proapoptotic conformation of Bak in a ΔMEKK1-dependent manner. Molecular and Cellular Biology 21, 3684-3691.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-August-2007 RFH
          SynonymsAnti-Bcl-2 Antagonist Killer
          ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence)
          Immunocytochemistry (see comments; see application references)
          Application Data
          Detection of human Bak by immunoblotting. Sample: Whole cell lysates from untreated HL-60 cells. Primary antibody: Anti-Bak (Ab-1) Mouse mAb (TC-100) (Cat. No. AM03) (2 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~30 (apparent MW) kDa Bak protein.
          BackgroundBak (for bcl-2 homologous antagonist/killer) is a member of the bcl-2 family of proteins. Bak was independently identified by three different groups using either the two hybrid system for identifying adenovirus E1B 19K interacting proteins or by PCR cloning using degenerate BH1 and BH2 domain primers derived from the human bcl-2 gene. Bak shows a 53% homology with bcl-2 in the BH1 and BH2 domains but an overall homology of 28% with bcl-2. The presence of a hydrophobic tail on the protein suggests that, like bcl-2, it is membrane localized. The Bak protein is translated from a 2.2 kb mRNA to yield a 211-216 amino acid protein with a predicted molecular weight of 23.4 kDa but migrates by gel electrophoresis as an approximately 30 kDa protein under denaturing conditions. The bak gene (bak-1) has been mapped to chromosome 6 and spans approximately 6 kb of DNA. Southern analysis has indicated the presence of two additional bak genes: bak-2 has 97% homology with bak-1 and maps to chromosome 20, and bak-3, a pseudogene, which maps to chromosome 11. Functionally, bak is similar to bax in that it induces apoptosis. The protein pairs with bcl-xL but unlike its pairing partner, has been found in most tissues tested. While conflicting reports exist for the heterodimerization of bak with other members of the bcl-2 family of proteins, it is clear that bak does partner with the E1B 19K protein.
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant human Bak from which the C-terminal and transmembrane domains have been deleted
          EpitopeWithin the N-terminal region
          CloneTC-100
          IsotypeIgG2a
          Specieshuman, not mouse, not rat
          Positive controlHL-60 cells
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThis antibody recognizes active/activated Bak under nondenaturing, native conditions using applications like immunocytochemistry (see application references). Antibody should be titrated for optimal results in individual systems for each application.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesChittenden, T., et al. 1995. Nature 374, 733.
          Farrow, S.N., et al. 1995. Nature 374, 731.
          Kiefer, M.C., et al. 1995. Nature 736.
          Reed, J.C. 1994. J. Cell Biol. 124, 1.
          Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
          Citation
        • Lena Thyrell, et al. (2004) Interferon α-induced apoptosis in tumor cells is mediated through the phosphoinositide 3-kinase/mammalian target of rapamycin signaling pathways. Journal of Biological Chemistry 279, 24152-24162.
        • Aleksandra Mandic, et al. (2001) Cisplatin induces the proapoptotic conformation of Bak in a ΔMEKK1-dependent manner. Molecular and Cellular Biology 21, 3684-3691.
        • Application referencesImmunocytochemistry Panaretakis, T., et al. 2002. J. Biol. Chem. 277, 44317.