A part of MilliporeSigma

MAB4132 | Anti-BRCA1 Antibody, clone BR64

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M IP, WB M Purified Monoclonal Antibody
      Catalogue NumberMAB4132
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-BRCA1 Antibody, clone BR64
      Product Information
      PresentationLiquid in phosphate buffer, containing azide as a preservative.
      ApplicationAnti-BRCA1 Antibody, clone BR64 is a high quality Mouse Monoclonal Antibody for the detection of BRCA1 & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation

      Western blot

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified polypeptide representing a 100-amino acid segment of the amino terminus of the BRCA1 gene product.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThe breast cancer susceptibility gene, BRCA1*, is located on chromosome 17 (at q21), and is mutated in the majority of heritable breast cancers. The mutations within the coding region occur throughout the gene with little apparent clustering. The majority of BRCA1 mutations result in shortened BRCA1 gene products, creating a need for an amino-terminal marker. Antibody MAB4132 recognizes both the full-length and mutated (shortened) forms of BRCA1 in breast cancer cell lines. It also recognizes over-expressed protein in transiently transfected Cos cells. A single cross-reacting protein of approximately 27 kDa is also seen upon immunoprecipitation. No other cross-reacting proteins are detected.

      BRAC1 is 220kDa.

      *BRCA1 is covered under U.S. patents 5,747,282, 5,753,441 and 6,162,897.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
      Gene Symbol
      • BRCA1
      • RNF53
      • BRCAI
      • PSCP
      • BRCC1
      • IRIS
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P38398 # Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Mediates E2-dependent ubiquitination. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
      SIZE: 1863 amino acids; 207721 Da
      SUBUNIT: Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. CTIP interacts specifically with the BRCT domains. Interacts with RNA polymerase II holoenzyme. Interacts with SMC1A and COBRA1/NELFB. Binds BRIP1 through the BRCT domains. Interacts with ubiquitinated FANCD2. Interacts with BAP1. Interacts with DCLRE1C/Artemis and CLSPN. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with CHEK1/CHK1. Interacts with BRCC3. Interacts through its BRCT domains with phosphorylated ACACA and prevents its dephosphorylation.
      DOMAIN: SwissProt: P38398 The RING-type zinc finger domain interacts with BAP1.
      PTM: Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
      DISEASE: "SwissProt: P38398 # Defects in BRCA1 are a cause of genetic susceptibility to breast cancer (BC) [MIM:113705, 114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination. & Defects in BRCA1 are a cause of genetic susceptibility to breast-ovarian cancer (BOC) [MIM:113705]. Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast- ovarian cancer. & Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705]."
      SIMILARITY: Contains 2 BRCT domains. & Contains 1 RING-type zinc finger.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-BRCA1 -2521467 2521467
      MOUSE ANTI-BRCA1 -2736485 2736485
      MOUSE ANTI-BRCA1 -2810620 2810620


      Reference overviewPub Med ID
      Mechanism of BRCA1-mediated inhibition of progesterone receptor transcriptional activity.
      Pragati Katiyar, Yongxian Ma, Anna Riegel, Saijun Fan, Eliot M Rosen, Pragati Katiyar, Yongxian Ma, Anna Riegel, Saijun Fan, Eliot M Rosen
      Molecular endocrinology (Baltimore, Md.) 23 1135-46 2009

      Show Abstract Full Text Article
      19389812 19389812
      Nuclear redistribution of BRCA1 during viral infection
      Maul, G. et al.
      Cell Growth & Differentiation, 9:743-755 (1998) 1998

      9751118 9751118

      Data Sheet

      Anti-BRCA1, clone BR64 - Data Sheet

      Related Products & Applications

      Related Products

      Catalog Number Description  
      07-434 Anti-BRCA1 Antibody Show Pricing & Availability
      07-635 Anti-phospho-BRCA1 (Ser1423) Antibody Show Pricing & Availability
      MABC161 Anti-BRCA1 Antibody, clone MS13 Show Pricing & Availability
      MABC199 Anti-BRCA1 Antibody, clone MS110 Show Pricing & Availability

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies