A part of MilliporeSigma

MABC535 | Anti-BIRC2/IAP-2, clone 1E1-1-10 Antibody

MABC535
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H WB R Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC535
      DescriptionAnti-BIRC2/IAP-2, clone 1E1-1-10 Antibody
      Alternate Names
      • Baculoviral IAP repeat-containing protein 2
      • Inhibitor of apoptosis protein 2
      • IAP-2
      • mIAP-2
      • mIAP2
      • cIAP1
      Background InformationBRC2/IAP-2, also known as IAP homolog B, Inhibitor of apoptosis protein 2 and Baculoviral IAP repeat-containing protein 2, and encoded by the gene BIRC2/API1/IAP2/MIHB/ RNF48, is a multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. BIRC2/IAP-2 also acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical pathways. Additionally, BIRC2/IAP-2 acts as an important regulator of innate immune signaling, and finally, BIRC2/IAP-2 plays an important role in the modulation of the cell cycle. BIRC2/IAP-21 is localized to nucleus except during apoptotic events. BIRC2/IAP-2 is expressed in fetal and adult tissues with a strong emphasis found in adult skeletal muscle, thymus, testis, ovary and pancreas with low or absent expression in blood and brain.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-BIRC2/IAP-2 antibody, clone 1E1-1-10 is an antibody against BIRC2/IAP-2 for use in western blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected BIRC2/IAP-2 in C2C12 cell lysate.

      Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in HeLa and Raji cell lysate (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in MEF cell lysate (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in stable cell lines expressing EYFP cIAP1 RING, or EYFP cIAP1 RING (Silke, J., et al. (2005). Proc Natl Acad Sci USA. 102(45):16182-16187.).
      Biological Information
      ImmunogenLinear peptide corresponding to the BIR 2 repeat region of mouse BIRC2/IAP-2.
      EpitopeThis antibody recognizes the BIR 2 repeat region of BIRC2/IAP-2.
      Clone1E1-1-10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Birc2
      • Birc3
      • lap2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~65 kDa observed. The calculated molecular weight of this protein is 70 kDa; however, this protein has been observed at lower molecular weights (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.; Silke, J., et al. (2005). Proc Natl Acad Sci USA. 102(45):16182-16187.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected BIRC2/IAP-2 in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-BIRC2/IAP-2, clone 1E1-1-10 - Q2385778 Q2385778
      Anti-BIRC2/IAP-2, clone 1E1-1-10 -2689657 2689657
      Anti-BIRC2/IAP-2, clone 1E1-1-10 -2720120 2720120

      References

      Reference overviewPub Med ID
      Linear ubiquitination prevents inflammation and regulates immune signalling.
      Gerlach, Björn, et al.
      Nature, 471: 591-6 (2011) 2011

      Show Abstract
      21455173 21455173
      Determination of cell survival by RING-mediated regulation of inhibitor of apoptosis (IAP) protein abundance.
      Silke, John, et al.
      Proc. Natl. Acad. Sci. U.S.A., 102: 16182-7 (2005) 2005

      Show Abstract
      16263936 16263936