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GF35L | Anti-BDNF Mouse mAb (35928.11)

GF35L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      GF35L-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
          Catalogue NumberGF35L
          Brand Family Calbiochem®
          SynonymsAnti-Brain Derived Neurotrophic Factor
          References
          ReferencesRiccio, A., et al. 1997. Science 277 1097.
          Sendtner, M., et al. 1996. Neurochem. Res. 21, 831.
          Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442.
          Gotz, R., et al. 1994. Nature 372, 266.
          Snider, W.D. 1994. Cell 77, 627.
          Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.
          Product Information
          FormLyophilized
          FormulationLyophilized from PBS, 5% trehalose.
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunohistochemistry
          Neutralization Studies
          Application NotesELISA (0.5-1 µg/ml)
          Immunoblotting (1-2 µg/ml)
          Immunohistochemistry (5-15 µg/ml)
          Neutralization Studies (see comments)
          Application CommentsThe full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant protein consisting of amino acids 129-247 of human BDNF
          ImmunogenHuman
          Clone35928.11
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          GF35L

          References

          Reference overview
          Riccio, A., et al. 1997. Science 277 1097.
          Sendtner, M., et al. 1996. Neurochem. Res. 21, 831.
          Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442.
          Gotz, R., et al. 1994. Nature 372, 266.
          Snider, W.D. 1994. Cell 77, 627.
          Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-April-2010 JSW
          SynonymsAnti-Brain Derived Neurotrophic Factor
          ApplicationELISA (0.5-1 µg/ml)
          Immunoblotting (1-2 µg/ml)
          Immunohistochemistry (5-15 µg/ml)
          Neutralization Studies (see comments)
          DescriptionImmunoaffinty purified mouse monoclonal antibody. Recognizes the ~20 kDa BDNF protein.
          BackgroundNeurotrophic factors define a family of related proteins, which are important in the development, maintenance, and regulation of neurons in both the peripheral and central nervous system. In addition to the prototypical nerve growth factor (NGF), several other factors have been identified which interact with a family of tyrosine kinase receptors. TrkA is the kinase signaling receptor for NGF, while TrkB mediates signaling for both brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5). TrkC is the primary receptor for neurotrophin-3 (NT-3), although this neurotrophin can also activate TrkA and TrkB receptors under certain conditions. Axotomy-induced cell death is one experimental paradigm that has been used to discriminate between the effects of various neurotrophic factors on the survival of different neuronal populations. BDNF, NT-3, and NT-4/5 act as motor neuron survival factors, whereas NGF has no effect on the survival of spinal cord motor neurons. NGF is neuroprotective for cholinergic neurons in the central nervous system. Retrogradely transported NGF-TrkA has also been shown to signal the CREB transcription factor in sympathetic neurons. A putative new member of this gene family, neurotrophin-6 (NT-6), has also been identified from the fish Xephophorus. Like NGF, NT-6 promotes the survival of sympathetic and sensory neurons, albeit with a lower potency.
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant protein consisting of amino acids 129-247 of human BDNF
          Clone35928.11
          IsotypeIgG₁
          Specieshuman
          FormLyophilized
          FormulationLyophilized from PBS, 5% trehalose.
          PreservativeNone
          CommentsThe full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesRiccio, A., et al. 1997. Science 277 1097.
          Sendtner, M., et al. 1996. Neurochem. Res. 21, 831.
          Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442.
          Gotz, R., et al. 1994. Nature 372, 266.
          Snider, W.D. 1994. Cell 77, 627.
          Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.