A part of MilliporeSigma

ABC973 | Anti-BAG3

100 μg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB Ch Affinity Purified Polyclonal Antibody
      Catalogue NumberABC973
      Alternate Names
      • BAG family molecular chaperone regulator 3
      • BAG-3
      • Bcl-2-associated athanogene 3
      • Bcl-2-binding protein Bis
      Background InformationBAG family molecular chaperone regulator 3 (UniProt Q9JLV1; also known as BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis) is encoded by the Bag3 (also known as Bis, MNCb-2243) gene (Gene ID 29810) in murine species. Bcl-2-associated athanogene 3 (BAG3) is a stress-activated protein abundantly expressed in the heart, skeletal muscles, and many cancers. BAG3 is involved in protein quality control (PQC) by serving as a co-chaperone for both constitutive and inducible heat shock proteins (Hsc/Hsp), as well as in mediating cellular anti-apoptotic activity via Bcl2 binding. BAG3 also plays a role in sarcomere stability by regulating filamin clearance and production, as well as by interacting with actin capping protein beta 1 (CapZβ1) that binds the barbed end of actin and prevent actinn fibers from disssociating into actin monomers. While BAG3 is found in the cytoplasm of neonatal myocytes and involved in PQC and apoptosis, BAG3 is localized predominantly in the sarcolemma and t-tubules of adult cardiac myocytes, where it modulates myocyte contraction in response to β-adrenergic receptor signaling. Bag3-knock mice die 4 weeks after birth due to non-inflammatory myofibrillar degeneration. Human BAG3 gene mutations are found in childhood-onset muscular dystrophy, in families with dilated cardiomyopathy, as well as in cases of idiopathic dilated cardiomyopathy.
      Product Information
      FormatAffinity Purified
      PresentationPurified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect BAG3 using this chicken polyclonal Anti-BAG3 Antibody, Cat. No. ABC973, validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding a sequence near the C-terminus of mouse BAG3.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityThis chicken polyclonal antibody detects mouse BAG3 by targeting a C-terminal region sequence. Rat species cross-reactivity is likely, but has not been confirmed.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Bag3
      • Bis
      • MNCb-2243
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~80 kDa observed. 61.73 kDa calculated (Met1 removed). the larger-than-calculated band size is consistent with that reported in the literature (Rasati, A., et al. (2015). Nat. Commun. 6:8695). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse heart tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected BAG3 in 10 µg of mouse heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information