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09-893 | Anti-BAF (BANF1) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB Rb Serum Polyclonal Antibody
      Catalogue Number09-893
      DescriptionAnti-BAF (BANF1) Antibody
      Alternate Names
      • 53 kDa BRG1-associated factor A
      • Actin-related protein Baf53a
      • BAF complex 53 kDa subunit
      • BRG1-associated factor
      • INO80 complex subunit K
      • actin-like 6A
      • actin-related protein 4
      • hArpN beta
      Background InformationBarrier-to-auto integration factor (BAF or BANF1) plays fundamental roles in nuclear assembly, chromatin organization, gene expression and development. BAF may potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. BAF contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. BAF is exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. BAF is found in both the nucleus and cytoplasm and is significantly enriched at the nuclear inner membrane, diffused throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase.
      Product Information
      • HeLa cell lysate
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      ApplicationUse Anti-BAF (BANF1) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect BAF (BANF1) also known as 53 kDa BRG1-associated factor A.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A 1:1,000 dilution from a previous lot detected BAF in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human BAF (BANF1).
      SpecificityThis antibody recognizes BAF (BANF1).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteProven to react with human, mouse, and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.
      Gene Symbol
      • ACTL6
      • ARPN-BETA
      • Actl6
      • Arp4
      • ArpNbeta
      • BAF53
      • BAF53A
      • Baf53a
      • INO80K
      • MGC5382
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodeling complex BAF with chromatin/nuclear matrix. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene By similarity.
      SUBUNIT STRUCTURE: Component of numerous complexes with chromatin remodeling and histone acetyltransferase activity. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and EAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400, and which may also include a HAT activity related to, but distinct from, that of KAT5. ACTL6A interacts with SMARCA4/BRG1. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.
      SEQUENCE SIMILARITIES: Belongs to the actin family.
      Molecular Weight~10 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.
      Western Blot Analysis: A 1:1,000 dilution of this antibody detected BAF in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-BAF (BANF1) 2470754
      Anti-BAF (BANF1) - 1969081 1969081
      Anti-BAF (BANF1) - 2211968 2211968
      Anti-BAF (BANF1) - 2227192 2227192
      Anti-BAF (BANF1) - 2343598 2343598
      Anti-BAF (BANF1) - NG1829867 NG1829867
      Anti-BAF (BANF1) - NRG1633609 NRG1633609
      Anti-BAF (BANF1) -2512460 2512460
      Anti-BAF (BANF1) -2575913 2575913
      Anti-BAF (BANF1) -2661539 2661539


      Reference overviewPub Med ID
      Systems analysis of the prostate tumor suppressor NKX3.1 supports roles in DNA repair and luminal cell differentiation.
      Yang, CC; Chung, A; Ku, CY; Brill, LM; Williams, R; Wolf, DA
      F1000Research 3 115 2014

      Show Abstract
      25177484 25177484

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