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MAB4500 | Anti-B23 Antibody, clone 3C9

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IF, IP, WB M Purified Monoclonal Antibody
      Catalogue NumberMAB4500
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-B23 Antibody, clone 3C9
      Alternate Names
      • Nucleophosmin
      Background InformationB23/Nucleophosmin is a major nucleolar phosphoprotein that is mainly located within the nucleolar granular compartment, where preribosomes are assembled. B23/Nucleophosmin is a multifunctional protein that is overexpressed in actively proliferating cells and cancer cells. Antibodies against B23 might be useful for recognition of human peripheral lymphocytes at early stages of their activation for proliferation, preceding the S-phase (Dergunova, 2002). By parallel immunolabeling with the anti-Ki-67 antibody, it was shown that the early changes of B23 amount and localization occurred before an appearance of Ki-67 protein, a well-known marker of proliferating cells (Dergunova, 2002).
      Product Information
      Presentation0.05M Potassium phosphate, 0.3M Sodium chloride, and 0.05% Sodium azide
      ApplicationAnti-B23 Antibody, clone 3C9 is an antibody against B23 for use in IF, IP & WB.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot



      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenTotal extracts of Daudi cell nuclei.
      SpecificityRecognizes human Nucleophosmin (B23).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NPM1
      • Numatrin
      • numatrin
      • Nucleophosmin
      • MGC104254
      • NPM
      • B23
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06748 # Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. It may function in the assembly and/or transport of ribosome.
      SIZE: 294 amino acids; 32575 Da
      SUBUNIT: Disulfide-linked dimers under certain conditions. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70 (By similarity). Interacts with NSUN2.
      SUBCELLULAR LOCATION: Nucleus, nucleolus. Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML.
      PTM: Phosphorylated.
      DISEASE: SwissProt: P06748 # A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. & A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA. & A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1. & Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.
      SIMILARITY: SwissProt: P06748 ## Belongs to the nucleoplasmin family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up 12 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-B23 MONOCLONAL ANTIBODY - 2092686 2092686
      MOUSE ANTI-B23 MONOCLONAL ANTIBODY - 2135104 2135104
      MOUSE ANTI-B23 MONOCLONAL ANTIBODY - 2433381 2433381
      MOUSE ANTI-B23 -2717668 2717668
      MOUSE ANTI-B23 -2733579 2733579
      MOUSE ANTI-B23 -2738432 2738432
      MOUSE ANTI-B23 MONOCLONAL ANTIBODY - 1983680 1983680
      MOUSE ANTI-B23 MONOCLONAL ANTIBODY - 2318787 2318787


      Reference overviewApplicationPub Med ID
      Major differences between tumor and normal human cell fates after exposure to chemotherapeutic monofunctional alkylator.
      Gupte, M; Tuck, AN; Sharma, VP; Williams, KJ
      PloS one 8 e74071 2013

      Show Abstract
      24019948 24019948
      ATRX-mediated chromatin association of histone variant macroH2A1 regulates α-globin expression.
      Ratnakumar, K; Duarte, LF; LeRoy, G; Hasson, D; Smeets, D; Vardabasso, C; Bönisch, C; Zeng, T; Xiang, B; Zhang, DY; Li, H; Wang, X; Hake, SB; Schermelleh, L; Garcia, BA; Bernstein, E
      Genes & development 26 433-8 2012

      Show Abstract
      Western Blotting22391447 22391447
      BRCA2 and nucleophosmin coregulate centrosome amplification and form a complex with the Rho effector kinase ROCK2.
      Wang, HF; Takenaka, K; Nakanishi, A; Miki, Y
      Cancer research 71 68-77 2011

      Show Abstract
      21084279 21084279
      Nucleolar disruption in dopaminergic neurons leads to oxidative damage and parkinsonism through repression of mammalian target of rapamycin signaling.
      Rieker, C; Engblom, D; Kreiner, G; Domanskyi, A; Schober, A; Stotz, S; Neumann, M; Yuan, X; Grummt, I; Schütz, G; Parlato, R
      The Journal of neuroscience : the official journal of the Society for Neuroscience 31 453-60 2011

      Show Abstract
      21228155 21228155
      Pten ablation in adult dopaminergic neurons is neuroprotective in Parkinson's disease models.
      Domanskyi, A; Geissler, C; Vinnikov, IA; Alter, H; Schober, A; Vogt, MA; Gass, P; Parlato, R; Schütz, G
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25 2898-910 2011

      Show Abstract
      21593433 21593433

      Data Sheet

      Anti-B23, clone 3C9 - Data Sheet

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