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MABE886 | Anti-B-Myb Antibody, clone LX015.1

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB M Purified Monoclonal Antibody
      Catalogue NumberMABE886
      DescriptionAnti-B-Myb Antibody, clone LX015.1
      Alternate Names
      • Myb-related protein B
      • B-Myb
      • Myb-like protein 2
      Background InformationB-Myb, also known as B-Myb or Myb-like protein 2, and encoded by the gene MYBL2/BMYB, is a transcription factor protein involved in the regulation of cell survival, proliferation and differentiation. B-Myb is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. B-Myb is a component of the DREAM complex (also named LINC complex) that is composed of at least E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2 proteins. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL22. B-Myb is localized to the nucleus. B-Myb is widely expressed and up regulated in cancer cells. B-Myb is an oncogenic transcription factor involved in G2/M progression and is a key target of anti-cancer therapy.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect B-Myb using this Anti-B-Myb Antibody, clone LX015.1 validated for use in western blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot from an independent laboratory detected U2OS cell lysates expressing B-Myb in U2OS (Chen, X., et al. (2013). Mol Cel Biol. 33(2):227-236.).

      Western Blotting Analysis: A representative lot from an independent laboratory in NIH/3T3 cell lysates and in undifferentiated and differentiated F9 cell lysates (Knight, A. S., et al. (2009). Oncogene. 28(15):1737-1747.).

      Western Blotting Analysis: A representative lot from an independent laboratory in cycling NIH/3T3 and F9 nuclear extracts (Muller, G. A., et al. (2012). Nucleic Acids Res. 40(4):1561-78.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected B-Myb wild-type cycling MEF cell lysates and not in B-Myb wild type G0 phase arrested MEF cell lysates. The same lot also detected B-Myb in G0 phase arrested and in cycling MEF cell lysates inappropriately expressing homozygous mutant B-Myb (Tavner, F., et al. (2007). Oncogene. 26(19): 2727-2735.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human B-Myb.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYBL2
      • BMYB
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~80 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1 μg/mL of this antibody detected B-Myb in 10 μg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-B-Myb, clone LX015.1 - Q2343948 Q2343948


      Reference overviewApplicationPub Med ID
      The forkhead transcription factor FOXM1 controls cell cycle-dependent gene expression through an atypical chromatin binding mechanism.
      Chen, Xi, et al.
      Mol. Cell. Biol., 33: 227-36 (2013) 2013

      Show Abstract
      Western Blotting23109430 23109430
      The CHR promoter element controls cell cycle-dependent gene transcription and binds the DREAM and MMB complexes.
      Müller, Gerd A, et al.
      Nucleic Acids Res., 40: 1561-78 (2012) 2012

      Show Abstract
      22064854 22064854
      A Lin-9 complex is recruited by B-Myb to activate transcription of G2/M genes in undifferentiated embryonal carcinoma cells.
      Knight, A S, et al.
      Oncogene, 28: 1737-47 (2009) 2009

      Show Abstract
      Western Blotting19252525 19252525
      Targeting an E2F site in the mouse genome prevents promoter silencing in quiescent and post-mitotic cells.
      Tavner, F, et al.
      Oncogene, 26: 2727-35 (2007) 2007

      Show Abstract
      17072340 17072340

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      Life Science Research > Antibodies and Assays > Primary Antibodies