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MABC91 | Anti-Atg18 (WIPI-2) Antibody, clone 2A2

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Purified Monoclonal Antibody
      Catalogue NumberMABC91
      DescriptionAnti-Atg18 (WIPI-2) Antibody, clone 2A2
      Alternate Names
      • WD repeat domain phosphoinositide-interacting protein 2
      • WIPI-2
      • WIPI49-like protein 2
      Background InformationAtg18, also known as WIPI-2, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.
      Product Information
      • Human testis tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-Atg18 (WIPI-2) Antibody, clone 2A2 is validated for use in WB, IH(P) for the detection of Atg18 (WIPI-2).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Atg18 in human colorectal adenocarcinoma tissue.
      Biological Information
      ImmunogenLinear peptide corresponding to the C-terminus of human Atg18.
      SpecificityThis antibody recognizes Atg18 at the C-terminus.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryWD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008].
      Gene Symbol
      • WIPI2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Probable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P.

      SUBCELLULAR LOCATION: Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side. Note: Enriched at preautophagosomal structure membranes in response to ptdIns3P.

      TISSUE SPECIFICITY: Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. SEQUENCE SIMILARITIES: Belongs to the WD repeat SVP1 family.

      Contains 3 WD repeats.
      Molecular Weight~49 kDa observed.
      6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human testis tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Atg18 (WIPI-2), clone 2A2 - 2274296 2274296
      Anti-Atg18 (WIPI-2), clone 2A2 - NRG1948886 NRG1948886
      Anti-Atg18 (WIPI-2), clone 2A2 -2665803 2665803
      Anti-Atg18 (WIPI-2), clone 2A2 -2790048 2790048
      Anti-Atg18 (WIPI-2), clone 2A2 -2802511 2802511
      Anti-Atg18 (WIPI-2), clone 2A2 -2816565 2816565


      Reference overviewApplicationPub Med ID
      WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
      Dooley, HC; Razi, M; Polson, HE; Girardin, SE; Wilson, MI; Tooze, SA
      Molecular cell 55 238-52 2014

      Show Abstract
      Immunocytochemistry24954904 24954904

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