178613 | Anti-Aquaporin 3 (275-292) Rabbit pAb

178613
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      178613-1ST
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          Plastic ampoule 1 st
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          Description
          OverviewRecognizes the ~40 kDa and ~29 kDa forms of aquaporin-3 in rat kidney membrane. Supplied with a control peptide (immunogen).
          Catalogue Number178613
          Brand Family Calbiochem®
          SynonymsAnti-AQP3, Anti-GLIP
          Application Data
          Detection of rat aquaporin 3 by immunoblotting. Sample: Extract from rat kidney membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (1:200). Detection: chemiluminescence.

          Detection of rat aquaporin 3 by immunohistochemistry. Samples: Longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (Dilution Assay Dependent). Detection: fluorescence.
          References
          ReferencesEchevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
          Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
          Product Information
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, 1% BSA, 5% sucrose, pH. 7.4 and 40 µg AQP3275-292 control peptide lyophilized from PBS.
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunohistochemistry Ishibashi, K., et al. 1997. Am. J. Physiol. 272, F235. Ecelbarger, C.A., et al. 1995. Am. J. Physiol. 269, F663.
          Key Applications Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesImmunoblotting (1:200)
          Immunohistochemistry (assay dependent; see application references)
          Application CommentsFor use as a negative control, preincubate 1 µg of peptide with 1 µg of antibody for 1 h at room temperature. The epitope is unique for AQP3 and is not present in other known related proteins. It is highly homologous in mouse (17/18 identical) and human (15/18 identical) AQP3 protein. Recognition of AQP3 can be fully inhibited with the soluble AQP3275-292 blocking peptide. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide [(KY)STEAENVKLAH(Nle)KHKEQI] corresponding to amino acids 275-292 of rat AQP3, with norleucine replacing methionine, conjugated to KLH
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the antibody with 50 µl deionized water to make a 300 µg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Reconstitue the peptide with 100 µl deionized water, aliquot, and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          178613

          References

          Reference overview
          Echevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
          Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-November-2009 RFH
          SynonymsAnti-AQP3, Anti-GLIP
          ApplicationImmunoblotting (1:200)
          Immunohistochemistry (assay dependent; see application references)
          Application Data
          Detection of rat aquaporin 3 by immunoblotting. Sample: Extract from rat kidney membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (1:200). Detection: chemiluminescence.

          Detection of rat aquaporin 3 by immunohistochemistry. Samples: Longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (Dilution Assay Dependent). Detection: fluorescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40 kDa and ~29 kDa aquaporin-3 proteins. Supplied with a control peptide (immunogen).
          BackgroundAQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It is also slightly permeable to urea and glycerol. AQP3 may play an important role in gastrointestinal tract water transport and in glycerol metabolism. AQP3 has a wide tissue distribution. In the kidney it is predominantly expressed in the inner medulla.
          HostRabbit
          Immunogen speciesRat
          Immunogena synthetic peptide [(KY)STEAENVKLAH(Nle)KHKEQI] corresponding to amino acids 275-292 of rat AQP3, with norleucine replacing methionine, conjugated to KLH
          IsotypeIgG
          Speciesrat
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, 1% BSA, 5% sucrose, pH. 7.4 and 40 µg AQP3275-292 control peptide lyophilized from PBS.
          Preservative≤0.1% sodium azide
          CommentsFor use as a negative control, preincubate 1 µg of peptide with 1 µg of antibody for 1 h at room temperature. The epitope is unique for AQP3 and is not present in other known related proteins. It is highly homologous in mouse (17/18 identical) and human (15/18 identical) AQP3 protein. Recognition of AQP3 can be fully inhibited with the soluble AQP3275-292 blocking peptide. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the antibody with 50 µl deionized water to make a 300 µg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Reconstitue the peptide with 100 µl deionized water, aliquot, and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesEchevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
          Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
          Application referencesImmunohistochemistry Ishibashi, K., et al. 1997. Am. J. Physiol. 272, F235. Ecelbarger, C.A., et al. 1995. Am. J. Physiol. 269, F663.