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AB742 | Anti-Apolipoprotein B Antibody

AB742
1 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IP, WB Gt Serum Polyclonal Antibody
      Description
      Catalogue NumberAB742
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Apolipoprotein B Antibody
      Alternate Names
      • ApoB
      References
      Product Information
      FormatSerum
      Control
      • Liver, HepG2 cells
      PresentationDefibrinated, delipidized and absorbed by solid phase chromatography as required. Goat antisera in 0.05M Tris-HCl pH 7.5, 0.5M NaCl with 0.1% sodium azide.
      Applications
      ApplicationAnti-Apolipoprotein B Antibody is an antibody against Apolipoprotein B for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot

      Immunoprecipitation

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified human Apolipoprotein B.
      HostGoat
      SpecificityApolipoprotein B. Monospecific by IEP with neat antibody vs. 2X pooled human serum and neat pooled human plasma.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination.
      Gene Symbol
      • APOB
      • apoB-48
      • FLDB
      • apoB-100
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04114 # Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
      SIZE: 4563 amino acids; 515563 Da
      SUBCELLULAR LOCATION: Secreted.
      PTM: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
      DISEASE: SwissProt: P04114 # Defects in APOB are a cause of familial hypobetalipoproteinemia (FHBL) [MIM:107730]. FHBL is a genetically heterogeneous autosomal co-dominant disorder, associated with reduced plasma concentrations of apoB, LDL and VLDL. Heterozygotes for FHBL are usually asymptomatic with LDL cholesterol and apoB- 100 concentrations less than 50% of those in normal plasma. Homozygotes have extremely low plasma LDL cholesterol and apoB-100 concentrations, and clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia [MIM:200100]. & Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]. FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. & Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.SIMILARITY:SwissProt: P04114 ## Contains 1 vitellogenin domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GOAT ANTI-APOLIPOPROTEIN B POLYCLONAL ANTIBODY - 2390374 2390374
      GOAT ANTI-APOLIPOPROTEIN B - 2492087 2492087
      GOAT ANTI-APOLIPOPROTEIN B -2519232 2519232
      GOAT ANTI-APOLIPOPROTEIN B -2573577 2573577
      GOAT ANTI-APOLIPOPROTEIN B -2592702 2592702
      GOAT ANTI-APOLIPOPROTEIN B -2668097 2668097
      GOAT ANTI-APOLIPOPROTEIN B -2722844 2722844
      GOAT ANTI-APOLIPOPROTEIN B -2788897 2788897
      GOAT ANTI-APOLIPOPROTEIN B POLYCLONAL ANTIBODY -2499546 2499546

      References | 16 Available | See All References

      Reference overviewPub Med ID
      Identification of protein disulfide isomerase 1 as a key isomerase for disulfide bond formation in apolipoprotein B100.
      Wang, S; Park, S; Kodali, VK; Han, J; Yip, T; Chen, Z; Davidson, NO; Kaufman, RJ
      Molecular biology of the cell 26 594-604 2015

      Show Abstract
      25518935 25518935
      The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.
      Fryer, LG; Jones, B; Duncan, EJ; Hutchison, CE; Ozkan, T; Williams, PA; Alder, O; Nieuwdorp, M; Townley, AK; Mensenkamp, AR; Stephens, DJ; Dallinga-Thie, GM; Shoulders, CC
      The Journal of biological chemistry 289 4244-61 2014

      Show Abstract
      24338480 24338480
      Erythrocyte-bound apolipoprotein B in relation to atherosclerosis, serum lipids and ABO blood group.
      Klop, B; van de Geijn, GJ; Bovenberg, SA; van der Meulen, N; Elte, JW; Birnie, E; Njo, TL; Janssen, HW; van Miltenburg, A; Jukema, JW; Cabezas, MC
      PloS one 8 e75573 2013

      Show Abstract
      24069429 24069429
      Exon skipping of hepatic APOB pre-mRNA with splice-switching oligonucleotides reduces LDL cholesterol in vivo.
      Disterer, P; Al-Shawi, R; Ellmerich, S; Waddington, SN; Owen, JS; Simons, JP; Khoo, B
      Molecular therapy : the journal of the American Society of Gene Therapy 21 602-9 2013

      Show Abstract
      23319054 23319054
      Opposing roles of cell death-inducing DFF45-like effector B and perilipin 2 in controlling hepatic VLDL lipidation.
      Li, X; Ye, J; Zhou, L; Gu, W; Fisher, EA; Li, P
      Journal of lipid research 53 1877-89 2012

      Show Abstract
      22661308 22661308
      Ubiquitination regulates the assembly of VLDL in HepG2 cells and is the committing step of the apoB-100 ERAD pathway.
      Fisher, EA; Khanna, NA; McLeod, RS
      Journal of lipid research 52 1170-80 2011

      Show Abstract
      21421992 21421992
      Lipoprotein profiles in SCID/uPA mice transplanted with human hepatocytes become human-like and correlate with HCV infection success.
      Steenbergen, RH; Joyce, MA; Lund, G; Lewis, J; Chen, R; Barsby, N; Douglas, D; Zhu, LF; Tyrrell, DL; Tyrrell, DL; Kneteman, NM
      American journal of physiology. Gastrointestinal and liver physiology 299 G844-54 2010

      Show Abstract
      20651006 20651006
      The AAA-ATPase p97 facilitates degradation of apolipoprotein B by the ubiquitin-proteasome pathway.
      Fisher, EA; Lapierre, LR; Junkins, RD; McLeod, RS
      Journal of lipid research 49 2149-60 2008

      Show Abstract
      18550891 18550891
      Antisense oligonucleotide-induced alternative splicing of the APOB mRNA generates a novel isoform of APOB.
      Khoo, B; Roca, X; Chew, SL; Krainer, AR
      BMC molecular biology 8 3 2007

      Show Abstract Full Text Article
      17233885 17233885
      Bile Acid Reduces the Secretion of Very Low Density Lipoprotein by Repressing Microsomal Triglyceride Transfer Protein Gene Expression Mediated by Hepatocyte Nuclear Factor-4
      Hirokane, H. et al.
      J Biol Chem., 279 (44):45685-45692 (2004) 2004

      15337761 15337761

      Data Sheet

      Title
      Anti-Apolipoprotein B - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies