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NB20 | Anti-AnkyrinG Mouse mAb (463)

NB20
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ca, H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      NB20-100UG
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~190 kDa ankyrinG protein in brain extracts. Reacts with all spliced forms of ankyrinG.
        • Antibody Target Gene Symbol: ANK3
        • Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
        • Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
        • Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)
        • Mu Entrez ID: 11735
        • Rat Entrez ID: 361833
        • Catalogue NumberNB20
          Brand Family Calbiochem®
          Application Data
          Detection of mouse AnkyrinG by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-AnkyrinG Mouse mAb (463) (Cat. No. NB20) (2 µg/ml) Detection: chemiluminescence.
          References
          ReferencesDavis, J.Q., et al. 1995. Mol. Biol. Cell 6, 177a, supplement.
          Kunimoto, M., et al. 1995. FEBS Lett. 357, 217.
          Lambert, S., et al. 1995. Mol. Biol. Cell 6, 98a supplement.
          Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol. 9, 27.
          Kunimoto, M., et al. 1991. J. Cell Biol. 115, 1319.
          Otto, E., et al. 1991. J. Cell Biol. 114, 241.
          Davis, J., and Bennett, V. 1984. J. Biol. Chem. 259, 13550.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Positive controlMouse or rat brain extract or unmyelinated axons
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesFrozen Sections (1-2 µg/ml)
          Immunoblotting (2-5 µg/ml)
          Immunofluorescence (1-2 µg/ml)
          Application CommentsAnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG
          ImmunogenHuman
          Clone463
          HostMouse
          Purity≥95% by SDS-PAGE
          IsotypeIgG₁
          Species Reactivity
          • Canine
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          NB20

          References

          Reference overview
          Davis, J.Q., et al. 1995. Mol. Biol. Cell 6, 177a, supplement.
          Kunimoto, M., et al. 1995. FEBS Lett. 357, 217.
          Lambert, S., et al. 1995. Mol. Biol. Cell 6, 98a supplement.
          Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol. 9, 27.
          Kunimoto, M., et al. 1991. J. Cell Biol. 115, 1319.
          Otto, E., et al. 1991. J. Cell Biol. 114, 241.
          Davis, J., and Bennett, V. 1984. J. Biol. Chem. 259, 13550.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-September-2007 RFH
          ApplicationFrozen Sections (1-2 µg/ml)
          Immunoblotting (2-5 µg/ml)
          Immunofluorescence (1-2 µg/ml)
          Application Data
          Detection of mouse AnkyrinG by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-AnkyrinG Mouse mAb (463) (Cat. No. NB20) (2 µg/ml) Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of AnkyrinG.
          BackgroundAnkyrins are a family of spectrin-binding proteins that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel, and the Na/K ATPase, as well as neuronal cell adhesion molecules related to L1. In the nervous system, three different ankyrins are known to exist; ankyrinB, ankyrinG, and ankyrinR. All three ankyrins contain an N-terminal membrane binding domain, an internal domain capable of binding spectrin, and a C-terminal regulatory domain which is the target of alternative splicing. AnkyrinB, the predominant neuronal ankyrin includes two isoforms of ~220 kDa and ~440 kDa. The ~220 kDa alternatively spliced isoform is the major form present in the adult brain, while the ~440 kDa form is preferentially expressed in the developing neonatal brain. Three forms of ankyrinG (~190 kDa, ~270 kDa, ~480 kDa) are localized in axonal initial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched. Ankyrins comprise ~0.5%-1% of the total membrane protein in the adult vertebrate brain.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG
          Clone463
          IsotypeIgG₁
          Speciescanine, human, mouse, rat
          Positive controlMouse or rat brain extract or unmyelinated axons
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥95% by SDS-PAGE
          Preservative≤0.1% sodium azide
          CommentsAnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesDavis, J.Q., et al. 1995. Mol. Biol. Cell 6, 177a, supplement.
          Kunimoto, M., et al. 1995. FEBS Lett. 357, 217.
          Lambert, S., et al. 1995. Mol. Biol. Cell 6, 98a supplement.
          Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol. 9, 27.
          Kunimoto, M., et al. 1991. J. Cell Biol. 115, 1319.
          Otto, E., et al. 1991. J. Cell Biol. 114, 241.
          Davis, J., and Bennett, V. 1984. J. Biol. Chem. 259, 13550.