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MAB4055 | Anti-Angiotensin Converting Enzyme Antibody, clone 3G8

MAB4055
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk ELISA, IP, ICC, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB4055
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Angiotensin Converting Enzyme Antibody, clone 3G8
      Alternate Names
      • ACE
      • CD143
      • DCP1
      • Peptidyl-dipeptidase A
      • Peptidase P
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in 100 mM borate buffer, pH 8.0 and 150 mM NaCl.
      Applications
      ApplicationDetect Angiotensin Converting Enzyme using this Anti-Angiotensin Converting Enzyme Antibody, clone 3G8 validated for use in ELISA, IP, IC, IH.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:200 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.

      Immunocytochemistry: 1:100. Reacts with endothelial cells, macrophages (activated) and THP1 cells (monocyte line).

      Immunoprecipitation: 1:1,000

      ELISA : 1:1,000

      Structure-function of ACE (Danilov et al., 1994)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenAngiotensin-converting enzyme from human lung
      Clone3G8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAngiotensin-converting enzyme [ACE] (N-terminal)
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.
      Gene Symbol
      • ACE
      • DCP
      • ACE1
      • carboxycathepsin
      • ACE-T
      • MGC26566
      • DCP1
      • CD143
      • EC 3.4.15.1
      • EC 3.2.1.- [Contains: Angiotensin-converting enzyme, testis- specific isoform, soluble form].
      • EC 3.4.15.1 [Contains: Angiotensin-converting enzyme, somatic isoform, soluble form].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P22966 # Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.| P12821 # Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator.
      COFACTOR: Binds 1 zinc ion per subunit. & Binds 2 chloride ions per subunit.
      SIZE: 732 amino acids; 83330 Da
      SUBCELLULAR LOCATION: Angiotensin-converting enzyme, testis- specific isoform, soluble form: Secreted. & Cell membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Spermatocytes, adult testis.
      DOMAIN: SwissProt: P22966
      PTM: Phosphorylated by CK2 on Ser-725; which allows membrane retention (By similarity).
      DISEASE: SwissProt: P12821 # Genetic variations in ACE may be a cause of susceptibility to ischemic stroke [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. & Defects in ACE are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). & Genetic variations in ACE can influence susceptibility to diabetic nephropathy [MIM:603933]. Diabetic nephropathy is a kidney disease and resultant kidney function impairment due to the long standing effects of diabetes on the microvasculature (glomerulus) of the kidney. Features include increased urine protein and declining kidney function.
      SIMILARITY: SwissProt: P22966 ## Belongs to the peptidase M2 family.| P12821 ## Belongs to the peptidase M2 family.
      MISCELLANEOUS: The glycosidase activity probably uses different active site residues than the metalloprotease activity. & Inhibitors of ACE are commonly used to treat hypertension and cardiac dysfunction.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Cellular distribution of angiotensin-converting enzyme after myocardial infarction.
      Falkenhahn, M, et al.
      Hypertension, 25: 219-26 (1995) 1995

      Show Abstract
      7531176 7531176
      Angiotensin converting enzyme expression is increased in small pulmonary arteries of rats with hypoxia-induced pulmonary hypertension.
      Morrell, N W, et al.
      J. Clin. Invest., 96: 1823-33 (1995) 1995

      Show Abstract
      7560074 7560074
      Structure-function analysis of angiotensin I-converting enzyme using monoclonal antibodies. Selective inhibition of the amino-terminal active site.
      Danilov, S, et al.
      J. Biol. Chem., 269: 26806-14 (1994) 1994

      Show Abstract
      7523412 7523412
      Immunohistochemical study of angiotensin-converting enzyme in human tissues using monoclonal antibodies.
      Danilov, S M, et al.
      Histochemistry, 87: 487-90 (1987) 1987

      Show Abstract
      2828286 2828286

      Data Sheet

      Title
      Anti-Angiotensin Converting Enzyme, clone 3G8 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies