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AB3120 | Anti-Angiopoietin-1 Antibody, NT

AB3120
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M ELISA, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB3120
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Angiopoietin-1 Antibody, NT
      Alternate Names
      • Ang-1
      Background InformationThe remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development. Angiopoietin-1 (mouse and human Ang-1; 498aa with 98% identity) is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic dvevelopment, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 appears to play a crucial role in mediating matrix and mesenchyme. It mediates blood vessel maturation and stability. Ang-1 may play a critical role in heart development. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496aa with 85% identity) may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Fetal heart
      PresentationAffinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
      Applications
      ApplicationThis Anti-Angiopoietin-1 Antibody, N-terminus is validated for use in ELISA, WB for the detection of Angiopoietin-1.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 ug/mL using ECL. Expected size of Ang-1 is ~55kDa.

      ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well (Catalog Number AG635).

      Immunohistochemistry: Not tested. It is recommended that the antibody be used at 2-20 ug/ml in formaldehyde fixed tissue.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen20 amino acid peptide sequence near the amino-terminus of mouse Ang-1.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes mouse Angiopoietin-1 (Ang-1). The immunogenic peptide sequence is 90% (18/20 aa) conserved in human Ang-1, 95% I pig and bovine, and has no significant sequence homology with Ang-2. Antibody crossreactivity in other species is not established.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAngiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
      Gene Symbol
      • ANGPT1
      • ANG1
      • ANG-1
      • AGP1
      • KIAA0003
      • Ang1
      • AGPT
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15389 # Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
      SIZE: 498 amino acids; 57513 Da
      SUBCELLULAR LOCATION: Secreted.
      PTM: Glycosylated.
      SIMILARITY: SwissProt: Q15389 ## Contains 1 fibrinogen C-terminal domain.
      MISCELLANEOUS: It may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart.
      Molecular Weight55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-ANGIOPOIETIN-1 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2142148 2142148
      RABBIT ANTI-ANGIOPOIETIN-1 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2433314 2433314
      RABBIT ANTI-ANGIOPOIETIN-1 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2459483 2459483
      RABBIT ANTI-ANGIOPOIETIN-1 AFFINITY-PURIFIED -2594848 2594848
      RABBIT ANTI-ANGIOPOIETIN-1 AFFINITY-PURIFIED -2728355 2728355
      RABBIT ANTI-ANGIOPOIETIN-1 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2049200 2049200
      RABBIT ANTI-ANGIOPOIETIN-1 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2246744 2246744
      RABBIT ANTI-ANGIOPOIETIN-1 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2296793 2296793

      References

      Reference overviewPub Med ID
      Increased Expression of Angiopoietins and Tie2 in the Lungs of Chronic Asthmatic Mice.
      Makinde TO, Agrawal DK
      Am J Respir Cell Mol Biol 2010

      Show Abstract
      20463289 20463289
      Sertoli Cell-Initiated Testicular Innate Immune Response through Toll-Like Receptor-3 Activation Is Negatively Regulated by Tyro3, Axl, and Mer Receptors.
      Sun B, Qi N, Shang T, Wu H, Deng T, Han D
      Endocrinology 2010

      Show Abstract
      20363878 20363878
      Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
      Dallabrida, SM; Ismail, NS; Pravda, EA; Parodi, EM; Dickie, R; Durand, EM; Lai, J; Cassiola, F; Rogers, RA; Rupnick, MA
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22 3010-23 2008

      Show Abstract Full Text Article
      18502941 18502941
      Conditional activation of FGFR1 in the prostate epithelium induces angiogenesis with concomitant differential regulation of Ang-1 and Ang-2.
      Winter, SF; Acevedo, VD; Gangula, RD; Freeman, KW; Spencer, DM; Greenberg, NM
      Oncogene 26 4897-907 2007

      Show Abstract
      17297442 17297442
      Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning.
      Davis, S, et al.
      Cell, 87: 1161-9 (1996) 1996

      8980223 8980223
      Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis.
      Suri, C, et al.
      Cell, 87: 1171-80 (1996) 1996

      8980224 8980224

      Data Sheet

      Title
      Anti-Angiopoietin-1, N-terminus - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies