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AB561 | Anti-Androgen Receptor Antibody

50 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ELISA, WB, IHC Rb Serum Polyclonal Antibody
      Catalogue NumberAB561
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Androgen Receptor Antibody
      Alternate Names
      • Dihydrotestosterone Receptor
      Product Information
      PresentationRabbit antiserum. Liquid containing 0.05% sodium azide.
      ApplicationUse Anti-Androgen Receptor Antibody (Rabbit Polyclonal Antibody) validated in ELISA, WB, IHC to detect Androgen Receptor also known as Dihydrotestosterone Receptor.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunoblotting: 1:1,000-1:5,000

      Immunohistochemistry on prostate tissues (Prins et al. 1991): 1:100-1:1,000

      ELISA: 1:10,000-1:50,000 using 50 -100 ng of control peptide (AG653)/well.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA synthetic peptide corresponding to the amino terminus of the rat and human androgen receptor (Lubahn, et al. 1988; Chang et al. 1988)).
      SpecificityAndrogen Receptor. Recognizes a band of approximately 108-110 kDa in rat ventral prostate nuclear extract and LNCap cells by Western blot.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.
      Gene Symbol
      • AR
      • AIS
      • DHTR
      • SMAX1
      • NR3C4
      • TFM
      • OTTHUMP00000061928
      • KD
      • HUMARA
      • SBMA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10275 # The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Activated, but not phosphorylated, by HIPK3.
      SIZE: 919 amino acids; 98989 Da
      SUBUNIT: Binds DNA as a homodimer. Part of a ternary complex containing AR, EFCAB6/DJBP and PARK7. Interacts with HIPK3 in the presence of androgen. The ligand binding domain interacts with MYST2/HBO1 in the presence of dihydrotestosterone. Interacts with EFCAB6/DJBP, PELP1, PQBP1, RANBP9, SPDEF, SRA1, TGFB1I1, ZNF318 and RREB1. The AR N-terminal poly-Gln region binds Ran resulting in enhancement of AR-mediated transactivation. Ran-binding decreases as the poly-Gln length increases. Interacts with ZMIZ1/ZIMP10 and ZMIZ2/ZMIP7 which both enhance its transactivation activity. Interacts with SLC30A9 (By similarity).
      DOMAIN: SwissProt: P10275 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
      PTM: Sumoylated on Lys-386 (major) and Lys-520. & Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-534 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression.
      DISEASE: SwissProt: P10275 # Defects in AR are the cause of androgen insensibility syndrome (AIS) [MIM:300068]; previously known as testicular feminization syndrome (TFM). It can be complete (CAIS) when external genitalia are phenotypically female; or partial (PAIS) when external genitalia are substantively ambiguous or mild (MAIS) when external genitalia are normal male or nearly so. & Defects in AR are the cause of X-linked spinal and bulbar muscular atrophy (SBMA) [MIM:313200]; also known as Kennedy disease. In SBMA patients the number of Gln ranges from 40 to 52. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. & Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor. & Defects in AR may be the cause of infertility male syndrome [MIM:308370]; also called androgen insensitivity. It is characterized by azoospermia, elevated testosterone and luteinizing hormone plasma levels and an abnormal androgen receptor. & Defects in AR are the cause of Reifenstein syndrome [MIM:312300]; also known as partial androgen insensitivity. The features of this form of male pseudohermaphroditism are hypospadias, hypogonadism, gynecomastia, normal XY karyotype, and a pedigree pattern consistent with X-linked recessive inheritance.
      SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites. & Transcriptional activity is enhanced by binding to RANBP9. & The level of tyrosine phosphorylation may serve as a diagnostic tool to predict patient outcome in response to hormone-ablation therapy. Inhibition of tyrosine phosphorylation may be an effective intervention target for hormone-refractory prostate cancer.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number


      Reference overviewPub Med ID
      Spermatogenesis arrest caused by conditional deletion of Hsp90α in adult mice.
      Kajiwara, C; Kondo, S; Uda, S; Dai, L; Ichiyanagi, T; Chiba, T; Ishido, S; Koji, T; Udono, H
      Biology open 1 977-82 2012

      Show Abstract
      23213375 23213375
      Yeast-based reporter assays for the functional characterization of cochaperone interactions with steroid hormone receptors.
      Heather A Balsiger, Marc B Cox, Heather A Balsiger, Marc B Cox, Heather A Balsiger, Marc B Cox
      Methods in molecular biology (Clifton, N.J.) 505 141-56 2009

      Show Abstract
      19117143 19117143

      Data Sheet

      Anti-Androgen Receptor - Data Sheet

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