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MABN639 | Anti-Amyloid βA4, clone 1E8 (Amino Terminus) Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC, IP M Affinity Purified Monoclonal Antibody
      Catalogue NumberMABN639
      DescriptionAnti-Amyloid βA4, clone 1E8 (Amino Terminus) Antibody
      Alternate Names
      • Amyloid beta A4 protein
      • ABPP
      • APPI
      • APP
      • Alzheimer disease amyloid protein
      • Cerebral vascular amyloid peptide
      • CVAP
      • PreA4
      • Protease nexin-II
      • PN-II
      Background InformationAmyloid beta A4 protein, also known as ABPP or APPI (APP) or Alzheimer disease amyloid protein or Cerebral vascular amyloid peptide (CVAP) or PreA4 or Protease nexin-II (PN-II), and encoded by the gene name APP or A4 or AD1, belongs to the APP family. The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59). Mab βA4N-1E8 recognizes the free N-terminus of the bA4 polypeptide with high preference and crossreacts with sAPPα.
      Product Information
      FormatAffinity Purified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS, PEG, Sucrose, and up to 0.1% sodium azide.
      ApplicationThis Anti-Amyloid βA4, clone 1E8 (Amino Terminus) Antibody is validated for use in western blotting, IHC & IP for the detection of Amyloid βA4.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot was used to detect Amyloid βA4 in human Alzheimer's brain and thalamus tissues.

      Western Blotting Analysis: A representative lot was used to detect Amyloid βA4 in Western Blotting. (Wiltfang, et al. 2001; Serneels, et al. 2009; Maler, et al. 2007).

      Immunoprecipitation Analysis: A representative lot was used to detect Amyloid βA4 in Immunoprecipitation. (Wiltfang, et al. 2001; Maler, et al. 2007).
      Biological Information
      ImmunogenKLH-conjugated peptide corresponding to the N-terminus of human Amyloid βA4.
      Concentration1 mg/mL
      SpecificityThis antibody is specific for the first 2 amino acids of the Amyloid beta peptide amino terminus.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodAffinity
      UniProt Number
      Molecular Weight~4 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human Alzheimer's brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Amyloid βA4 in human Alzheimer's brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.

      Avoid repeated freeze / thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Amyloid βA4, clone 1E8 (Amino Terminus) - QVP1401280 QVP1401280
      Anti-Amyloid βA4, clone 1E8 (Amino Terminus) - VP1401230 VP1401230


      Reference overviewPub Med ID
      Retromer disruption promotes amyloidogenic APP processing.
      Sullivan, CP; Jay, AG; Stack, EC; Pakaluk, M; Wadlinger, E; Fine, RE; Wells, JM; Morin, PJ
      Neurobiology of disease 43 338-45 2011

      Show Abstract
      21515373 21515373
      gamma-Secretase heterogeneity in the Aph1 subunit: relevance for Alzheimer's disease.
      Serneels, L; Van Biervliet, J; Craessaerts, K; Dejaegere, T; Horré, K; Van Houtvin, T; Esselmann, H; Paul, S; Schäfer, MK; Berezovska, O; Hyman, BT; Sprangers, B; Sciot, R; Moons, L; Jucker, M; Yang, Z; May, PC; Karran, E; Wiltfang, J; D'Hooge, R; De Strooper, B
      Science (New York, N.Y.) 324 639-42 2009

      Show Abstract
      19299585 19299585
      Urea-based two-dimensional electrophoresis of beta-amyloid peptides in human plasma: evidence for novel Abeta species.
      Maler, JM; Klafki, HW; Paul, S; Spitzer, P; Groemer, TW; Henkel, AW; Esselmann, H; Lewczuk, P; Kornhuber, J; Wiltfang, J
      Proteomics 7 3815-20 2007

      Show Abstract
      17924594 17924594
      Highly conserved and disease-specific patterns of carboxyterminally truncated Abeta peptides 1-37/38/39 in addition to 1-40/42 in Alzheimer's disease and in patients with chronic neuroinflammation.
      Wiltfang, J; Esselmann, H; Bibl, M; Smirnov, A; Otto, M; Paul, S; Schmidt, B; Klafki, HW; Maler, M; Dyrks, T; Bienert, M; Beyermann, M; Rüther, E; Kornhuber, J
      Journal of neurochemistry 81 481-96 2002

      Show Abstract
      12065657 12065657
      Elevation of beta-amyloid peptide 2-42 in sporadic and familial Alzheimer's disease and its generation in PS1 knockout cells.
      Wiltfang, J; Esselmann, H; Cupers, P; Neumann, M; Kretzschmar, H; Beyermann, M; Schleuder, D; Jahn, H; Rüther, E; Kornhuber, J; Annaert, W; De Strooper, B; Saftig, P
      The Journal of biological chemistry 276 42645-57 2001

      Show Abstract
      11526104 11526104