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ABE304 | Anti-Alpha-Actinin-4 Antibody

ABE304
100 µL  
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      Overview

      Replacement Information
      Replacement InformationMABT144 is the recommended replacement for Discontinued catalog number ABE304

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, ChIP Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberABE304
      DescriptionAnti-Alpha-Actinin-4 Antibody
      Alternate Names
      • Alpha-actinin-4
      • F-actin cross-linking protein
      • Non-muscle alpha-actinin 4
      Background InformationAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

      ACTN4 contains a functional LXXLL receptor interaction motif that is reported to interact with nuclear receptors in vitro and in mammalian cells, which potently activates nuclear receptor-mediated transcription. It is shown that overexpression of ACTN4 potentiates estrogen receptor alpha-mediated transcription in transient transfection reporter assays, whereas knockdown of ACTN4 or HDAC7 expression decreases it. Additionally, mutant ACTN4, which lack the CaM (calmodulin)-like
      domain required for HDAC7 interaction, fail to activate ER alpha-mediated transcription.
      References
      Product Information
      FormatSerum
      Control
      • A431 cell lysate
      PresentationRabbit polyclonal in serum with 0.05% sodium azide.
      Applications
      ApplicationDetect Alpha-Actinin-4 using this Anti-Alpha-Actinin-4 Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Chakraborty, S., et al. (2006). J Biol Chem. 281(46):35070-35080.)

      Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa and CV-1 cells. (Chakraborty, S., et al. (2006). J Biol Chem. 281(46):35070-35080.)

      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in MCF-7 cells. (Khurana, S., et al. (2006). J Biol Chem. 286(3):1850-1859.)
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human ACTN4.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
      Gene Symbol
      • ACTN4
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

      SUBUNIT STRUCTURE: Homodimer; antiparallel By similarity. Binds TRIM3 at the N-terminus. Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with BAIAP1 and PDLIM2.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF7, oral floor cancer IMC2, and bladder cancer KU7).
      Molecular Weight~105 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected ACTN4 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies