MAB1012 Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only

MAB1012
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      E. coli IP, WB, ICC M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB1012
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Alkaline Phosphatase Antibody, E. coli, bacterial only
      Alternate Names
      • AP
      • Alk. Phos.
      References
      Product Information
      FormatAscites
      PresentationAscites. Contains no preservative.
      Applications
      ApplicationAnti-Alkaline Phosphatase Antibody, E. coli, bacterial only detects level of Alkaline Phosphatase & has been published & validated for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern blot at 1:5,000. 42-45kDa on SDS-PAGE,reducing gels for natural E. coli alkaline phosphatase monomer. Alkaline phosphatase fusion proteins will vary depending upon the target fusion protein.

      Immunocytochemistry: reacts with E.coli. AP fusion protein targets in acetone fixed cell preparations. 1:4000, other fixatives or conditions untested.

      ASSAY:

      Preparation of E. coli TnphoA transformants: E. coli strain CC118 was transformed with plasmid pGEM-3Z containing TnphoA insertional mutations in the p101 gene of Mycoplasma hyorhinis, which encodes a protein with a typical N-terminal prokaryotic single peptide (Yogev et al. 1991).

      Identification of fusion protein with MAB1012 transformants: Transformants are grown in 2XYT medium to OD600=0.6. Cells were centrifuged 3 minutes at 10,000 x g, suspended in SDS-PAGE sample buffer, heated at 100°C for 5 minutes, frozen and thawed and centrifuged as above at room temperature to remove insoluble material. The sample is applied at 9% to a SDS-PAGE gel, and Western immunoblot is performed as described (Yogev et al. 1991).

      Immunoprecipitation: 5μL of antibody per 500μL of lysate in RIPA or 0.5% triton X-100 solutions.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenF201C1B
      EpitopeE. coli, bacterial only
      HostMouse
      SpecificityAlkaline phosphatase (AP). MAB1012 has a high affinity and recognizes an AP determinant resistant to denaturation by SDS-PAGE. It is therefore ideally suited for sensitive and specific detection of AP fusion proteins by Western blot analysis of E. coli transformants expressing fusion products.
      IsotypeIgG2a
      Species Reactivity
      • E. coli
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAlkaline phosphatases are non-specific phosphomonoesterases that catalyze the hydrolysis reaction via a phosphoseryl intermediate to produce inorganic phosphate and the corresponding alcohol, optimally at high pH. Alkaline phosphatase exists as a dimer, each monomer binding 2 zinc atoms and one magnesium atom, which are essential for enzymatic activity.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00634# alkaline phosphatase activity
      COFACTOR: Binds 1 magnesium ion; Binds 2 zinc ions.
      SIZE: 471 amino acids; 49,439 Da
      SUBUNIT: Homodimer.
      SUBCELLULAR LOCATION: Periplasm, Periplasmic space.
      PTM: Disulfide bond; Phosphoprotein.
      SIMILARITY: SwissProt: P00634 ## Belongs to the alkaline phosphatase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI)2465147
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE MONOCLONAL ANTIBODY - 21399652139965
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE MONOCLONAL ANTIBODY - 23700952370095
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE - 29854252985425
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE -25610022561002
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE -25840392584039
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE -27364372736437
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE MONOCLONAL ANTIBODY3112634
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE MONOCLONAL ANTIBODY2920355
      MOUSE ANTI-ESCHERICHIA COLI (E. COLI) ALKALINE PHOSPHATASE MONOCLONAL ANTIBODY - 22395862239586

      References

      Reference overviewPub Med ID
      Both the stroma and thylakoid lumen of tobacco chloroplasts are competent for the formation of disulphide bonds in recombinant proteins.
      Julia Bally,Eric Paget,Michel Droux,Claudette Job,Dominique Job,Manuel Dubald
      Plant biotechnology journal  6  2008

      Show Abstract
      17944820 17944820
      Functional and topological analysis of the Burkholderia cenocepacia priming glucosyltransferase BceB, involved in the biosynthesis of the cepacian exopolysaccharide.
      Videira, PA; Garcia, AP; Sá-Correia, I
      Journal of bacteriology  187  5013-8  2005

      Show Abstract
      15995219 15995219
      Identification of Mycobacterium avium DNA sequences that encode exported proteins by using phoA gene fusions.
      J D Carroll, R C Wallace, J Keane, H G Remold, R D Arbeit, J D Carroll, R C Wallace, J Keane, H G Remold, R D Arbeit
      Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease  80  117-30  2000

      Show Abstract
      10970760 10970760
      Sequence and TnphoA analysis of a Mycoplasma hyorhinis protein with membrane export function.
      Yogev, D, et al.
      J. Bacteriol., 173: 2035-44 (1991)  1991

      Show Abstract
      1848219 1848219
      Alkaline phosphatase fusions: sensors of subcellular location.
      Manoil, C, et al.
      J. Bacteriol., 172: 515-8 (1990)  1990

      Show Abstract
      2404939 2404939
      A genetic approach to analyzing membrane protein topology.
      Manoil, C and Beckwith, J
      Science, 233: 1403-8 (1986)  1986

      Show Abstract
      3529391 3529391
      TnphoA: a transposon probe for protein export signals.
      Manoil, C and Beckwith, J
      Proc. Natl. Acad. Sci. U.S.A., 82: 8129-33 (1985)  1985

      Show Abstract
      2999794 2999794
      Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.
      Hoffman, C S and Wright, A
      Proc. Natl. Acad. Sci. U.S.A., 82: 5107-11 (1985)  1985

      Show Abstract
      3860846 3860846

      Data Sheet

      Title
      Anti-Alkaline Phosphatase, E. coli, bacterial only - Data Sheet