07-372 | Anti-Akt2/PKBβ Antibody

07-372
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IP, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-372
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Akt2/PKBβ Antibody
      References
      Product Information
      FormatPurified
      Control
      • L6 cell lysate
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationThis Anti-Akt2/PKBβ Antibody is validated for use in IP, WB for the detection of Akt2/PKBβ.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogenpeptide (C-RYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBb
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Akt2/PKBb; does not cross-react with Akt1 or Akt3.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
      Gene Symbol
      • AKT2
      • RAC-BETA
      • PRKBB
      • PKBBETA
      • RAC-PK-beta
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31751 # General protein kinase capable of phosphorylating several known proteins.
      SIZE: 481 amino acids; 55769 Da
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DISEASE:SwissProt: P31751 # Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.
      SIMILARITY: SwissProt: P31751 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from rat L6 skeletal muscle cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Akt2/PKB#946; (rabbit polyclonal IgG) - 2123173 2123173
      Anti-Akt2/PKB#946; (rabbit polyclonal IgG) - 2145426 2145426
      Anti-Akt2/PKB#946; (rabbit polyclonal IgG) - NG1830454 NG1830454
      Anti-Akt2/PKBb (rabbit polyclonal IgG) - 2316794 2316794
      Anti-Akt2/PKBb - JBC1873440 JBC1873440
      Anti-Akt2/PKBbeta; - 22444 22444
      Anti-Akt2/PKBbeta; - 27551 27551
      Anti-Akt2/PKBβ (rabbit polyclonal IgG) -2504292 2504292
      Anti-Akt2/PKBβ - 2542051 2542051
      Anti-Akt2/PKBβ -2591926 2591926

      References

      Reference overviewApplicationPub Med ID
      Dose-dependent effects of sirolimus on mTOR signaling and polycystic kidney disease.
      Novalic, Z; van der Wal, AM; Leonhard, WN; Koehl, G; Breuning, MH; Geissler, EK; de Heer, E; Peters, DJ
      Journal of the American Society of Nephrology : JASN  23  842-53  2012

      Show Abstract
      Western Blotting22343118 22343118
      The Akt isoforms are present at distinct subcellular locations.
      Santi, SA; Lee, H
      American journal of physiology. Cell physiology  298  C580-91  2010

      Show Abstract
      20018949 20018949
      Phosphorylation of PRAS40 on Thr246 by PKB/AKT facilitates efficient phosphorylation of Ser183 by mTORC1.
      Nascimento EB, Snel M, Guigas B, van der Zon GC, Kriek J, Maassen JA, Jazet IM, Diamant M, Ouwens DM
      Cell Signal  22  961-7. Epub 2010 Feb 6.  2010

      Show Abstract
      20138985 20138985
      Effects of endurance exercise training on insulin signaling in human skeletal muscle: interactions at the level of phosphatidylinositol 3-kinase, Akt, and AS160.
      Frøsig, C; Rose, AJ; Treebak, JT; Kiens, B; Richter, EA; Wojtaszewski, JF
      Diabetes  56  2093-102  2007

      Show Abstract
      Western Blotting17513702 17513702
      Exercise reverses high-fat diet-induced impairments on compartmentalization and activation of components of the insulin-signaling cascade in skeletal muscle.
      Yaspelkis, BB; Lessard, SJ; Reeder, DW; Limon, JJ; Saito, M; Rivas, DA; Kvasha, I; Hawley, JA
      American journal of physiology. Endocrinology and metabolism  293  E941-9  2007

      Show Abstract
      Western Blotting17623749 17623749
      Use of RNA interference-mediated gene silencing and adenoviral overexpression to elucidate the roles of AKT/protein kinase B isoforms in insulin actions
      Katome, T., et al
      J Biol Chem, 278:28312-23 (2003)  2003

      Immunoblotting (Western)12734182 12734182

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies