A part of MilliporeSigma

16-318 | Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate

200 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IP M Purified Monoclonal Antibody
      Catalogue Number16-318
      DescriptionAnti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate
      Background InformationAkt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.
      Product Information
      • NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
      Presentation20% slurry; 1X PBS, 0.05% sodium azide.
      Sufficient for 10 IPs.
      ApplicationDetect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.
      Key Applications
      • Immunoprecipitation
      Biological Information
      ImmunogenGST-fusion protein corresponding to residues 1-149 of human Akt1
      Cloneclone SKB1
      SpecificityRecognizes Akt1/PKBα.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Chromatography
      Molecular Weight~ 60 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information