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MABE911 | Anti-Aiolos Antibody, clone 9D10

MABE911
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ChIP-seq, ChIP M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE911
      DescriptionAnti-Aiolos Antibody, clone 9D10
      Alternate Names
      • Zinc finger protein Aiolos
      • Ikaros family zinc finger protein 3
      Background InformationAiolos is a zinc finger DNA binding transcription factor important in lymphocyte differentiation and development. Aiolos plays an essential role in the regulation of B-cell differentiation including, proliferation and maturation to plasma cells. Aiolos also works as a cofactor in gene suppression via its interaction with the transcription factor FOXP3 in regulatory T cells as well, and Aiolos is involved in various chromatin functions too through its interaction with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A. Aiolos is localized in the nucleus primarily but can be found in the cytoplasm depending upon the cellular situation. Aiolos is expressed in leukocytes and found in high concentration in spleen and thymus and has multiple isoforms. Activity is regulated via phosphorylation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Aiolos Antibody, clone 9D10 is validated for use in Western Blotting and ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of Aiolos.
      Key Applications
      • Western Blotting
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).

      ChIP-seq: A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).

      Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in an HL-60 cell lysate
      Biological Information
      ImmunogenRecombinant protein corresponding to human Aiolos.
      Clone9D10
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IKZF3
      • ZNFN1A3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~ 58-70 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in a Jurkat cell lysate.

      Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in 10 µg of a Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Aiolos, clone 9D10 Q2471545
      Anti-Aiolos, clone 9D10 -2626787 2626787
      Anti-Aiolos, clone 9D10 -2643629 2643629
      Anti-Aiolos, clone 9D10 -2662349 2662349
      Anti-Aiolos, clone 9D10 -2728873 2728873

      References

      Reference overviewPub Med ID
      Harnessing of the nucleosome-remodeling-deacetylase complex controls lymphocyte development and prevents leukemogenesis.
      Zhang, J; Jackson, AF; Naito, T; Dose, M; Seavitt, J; Liu, F; Heller, EJ; Kashiwagi, M; Yoshida, T; Gounari, F; Petrie, HT; Georgopoulos, K
      Nature immunology 13 86-94 2012

      Show Abstract
      22080921 22080921