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MABE253 | Anti-Ago2 Antibody, clone 11A9

MABE253
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IP, ICC R Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE253
      DescriptionAnti-Ago2 Antibody, clone 11A9
      Alternate Names
      • Protein argonaute-2
      • Argonaute2
      • hAgo2
      • Eukaryotic translation initiation factor 2C 2
      • eIF-2C 2
      • eIF2C 2
      • PAZ Piwi domain protein
      • PPD
      • Protein slicer
      Background InformationAgo2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Ago2 Antibody, clone 11A9 (Rat Monoclonal Antibody) validated in WB, IP, ICC to detect Ago2 also known as Protein argonaute-2, Argonaute2, hAgo2, eIF-2C 2.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory detected Ago2 in HEK239 cell lysate (Rudel, S., et al. (2008) RNA. 14(6):1244-1253.).

      Immunocytochemistry Analysis: A represeentative lot from an independent laboratory detected Ago2 in HEK293, HeLa, and RPE-1 cells (Weinmann, L., et al. (2009). Cell. 136(3):496-507.).
      Biological Information
      ImmunogenLinear peptide corresponding to human Ago2.
      Clone11A9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EIF2C2
      • AGO2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~85 kDa observed. Two isoforms at ~97 kDa and ~94 kDa are known to exist.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: A 1:500 dilution of this antibody detected Ago2 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Ago2, clone 11A9 2480963
      Anti-Ago2, clone 11A9 - 2135146 2135146
      Anti-Ago2, clone 11A9 - 2398927 2398927
      Anti-Ago2, clone 11A9 - 2432504 2432504
      Anti-Ago2, clone 11A9 - 2465284 2465284
      Anti-Ago2, clone 11A9 - 2276414 2276414
      Anti-Ago2, clone 11A9 - Q2032353 Q2032353
      Anti-Ago2, clone 11A9 -2524846 2524846
      Anti-Ago2, clone 11A9 -2552635 2552635
      Anti-Ago2, clone 11A9 -2702169 2702169
      Anti-Ago2, clone 11A9 -2725214 2725214
      Anti-Ago2, clone 11A9 -2776622 2776622
      Anti-Ago2, clone 11A9 -2794955 2794955

      References

      Reference overviewPub Med ID
      MicroRNA-122 dependent binding of Ago2 protein to hepatitis C virus RNA is associated with enhanced RNA stability and translation stimulation.
      Conrad, KD; Giering, F; Erfurth, C; Neumann, A; Fehr, C; Meister, G; Niepmann, M
      PloS one 8 e56272 2013

      Show Abstract
      23405269 23405269
      EBV and human microRNAs co-target oncogenic and apoptotic viral and human genes during latency.
      Riley, KJ; Rabinowitz, GS; Yario, TA; Luna, JM; Darnell, RB; Steitz, JA
      The EMBO journal 31 2207-21 2012

      Show Abstract
      22473208 22473208
      Characterization of viral and human RNAs smaller than canonical MicroRNAs.
      Li, Z; Kim, SW; Lin, Y; Moore, PS; Chang, Y; John, B
      Journal of virology 83 12751-8 2009

      Show Abstract
      19812168 19812168
      A multifunctional human Argonaute2-specific monoclonal antibody.
      Rüdel, Sabine, et al.
      RNA, 14: 1244-53 (2008) 2008

      Show Abstract
      18430891 18430891

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies