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ABS516 | Anti-Adiponectin

NewABS516
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M WB, IHC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABS516
      ReplacesAB3276P
      DescriptionAnti-Adiponectin
      Alternate Names
      • Adiponectin
      • 30 kDa adipocyte complement-related protein
      • ACRP30
      • Adipocyte complement-related 30 kDa protein
      • Adipocyte
      • C1q and collagen domain-containing protein
      • Adipose most abundant gene transcript 1 protein
      • apM-1
      • Gelatin-binding protein
      Background InformationAdiponectin (UniProt Q8K3R4; also known as 30 kDa adipocyte complement-related protein, ACRP30, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, apM-1, Gelatin-binding protein) is encoded by the Adipoq (also known as Acdc, Acrp30) gene (Gene ID 246253) in rat. Adipokines are adipose tissue-secreted factors involved in vascular function and metabolic disease. While most adipokines are proinflammatory and directly responsible for the development of the chronic inflammatory state in obesity, adiponectin is an adipokine with anti-inflammatory and insulin-sensitizing properties. Adiponectin mediates it biological activity via adiponection receptor protein 1 & 2 (AdipoR1 & AdipoR2). Both AdipoR1 and AdipoR2 are reported to be ubiquitously expressed with the highest expression in skeletal muscle and liver, respectively. Circulating adiponectin levels are reduced in obesity and type II diabetes, whereas weight loss elevates serum adiponectin. Likewise, adiponectin overexpression is reported to alleviate metabolic dysfunction independent of weight loss in both genetic and diet-induced animal models of obesity. Aadiponectin deficiency is associated with various clinical cardiovascular disorders including myocardial infarction, peripheral artery disease, and endothelial dysfunction. Adiponectin is produced with a signal peptide sequence (a.a. 1-18 in human and rat sequence; a.a. 1-17 in mouse), which is removed postranslationally to yield the mature protein with a collagen-like domain (a.a. 42-107 in human and rat, a.a. 45-110 in mouse) and a C1q domain (a.a. 108-244 in human and rat, a.a. 111-247 in mouse).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect Adiponectin using this rabbit polyclonal Anti-Adiponectin Antibody, Cat. No. ABS516, validated for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Adiponectin in rat cardiac muscle and kidney tissue sections.

      This polyclonal antibody is also available as Alexa Fluor® 488 conjugate (Cat. No. ABS516-AF488) for direct immunofluorescence staining.
      Biological Information
      ImmunogenGST-tagged recombinant rat adiponectin collagen-like domain.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody was raised against rat adiponectin collagen-like domain and validated for target band detection in both rat and mouse serum samples by Western blotting. Human and Rat adiponectins share 82% sequence identity in the collagen-like domain.
      Species Reactivity
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Adipoq
      • Acdc
      • Acrp30
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~33 kDa observed. Target band size appears larger than the calculated molecular weights of 24.92/26.81 kDa (mouse mature/pro-form) and 26.41/24.41 kDa (rat mature/pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat serum.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Adiponectin in 10 µg of rat serum protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications