DP11 Anti-Adenovirus 2 E1A Mouse mAb (M73)

DP11
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Adenovirus Infected Cells M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DP11-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the adenovirus 2 E1A and 5 E1A proteins, immunoprecipitating 3 proteins at ~30-35 kDa, as well as associated proteins such as Rb, p53, and cyclins.
          Catalogue NumberDP11
          Brand Family Calbiochem®
          References
          ReferencesWhyte, P., et al. 1998. Nature 334, 124.
          Ziff, E. et al. 1985. Cell 40, 705.
          Houweling, A., et al. 1980. Virology 105, 537.
          Berk, A.J., et al. 1979. Cell 17, 935.
          Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
          Shiroki, K., et al. 1979. Virology 95, 127.
          Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
          Graham, F.L., et al. 1974. Cold Spring Harbor Symp. Quant. Biol. 39, 637.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
          Negative controlHS27 cells
          Positive controlHEK293 cells or adenovirus-infected tissue
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoblotting Stiewe T., et al. 2000. Cancer Res. 60, 3957. Nishihara, A., et al. 1999. J. Biol. Chem. 274, 28716. Sanchez-Prieto, R., et al. 1999. Nat. Med. 5, 1076. van den Berg, A., et al. 2005. Respir. Res. 6, 111. Immunocytochemistry van den Berg, A., et al. 2005. Respir. Res. 6, 111. Immunofluoresecence Harlow, E., et al. 1986. Mol. Cell. Biol. 6, 1579. Harlow, E., et al. 1985. J. Virol. 3, 533. Original Clone Harlow, E., et al. 1985. J. Virol. 3, 533. Paraffin Sections Nemunaitis, H., et al. 2000 Cancer Res. 60, 6359.
          Key Applications Immunofluorescence
          Immunoprecipitation
          Paraffin Sections
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesImmunofluorescence (2-5 µg/ml, see application references)
          Immunoprecipitation (1-2 µg antibody/mg total protein)
          Immunoblotting (1 µg/ml; see application references)
          Immunocytochemistry (see application references)
          Frozen Sections (2-4 µg/ml)
          Paraffin Sections (2-4 µg/ml; heat pre-treatment required; see application references)
          Application CommentsImmunoprecipitates the adenovirus 2 and 5 E1A proteins (3 bands at ~30-50 kDa) as well as associated proteins. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenadenovirus 2 E1A protein
          CloneM73
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Adenovirus Infected Cells
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Adenovirus 2 E1A Mouse mAb (M73) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Adenovirus 2 E1A Mouse mAb (M73) Certificates of Analysis

          TitleLot Number
          DP11

          References

          Reference overview
          Whyte, P., et al. 1998. Nature 334, 124.
          Ziff, E. et al. 1985. Cell 40, 705.
          Houweling, A., et al. 1980. Virology 105, 537.
          Berk, A.J., et al. 1979. Cell 17, 935.
          Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
          Shiroki, K., et al. 1979. Virology 95, 127.
          Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
          Graham, F.L., et al. 1974. Cold Spring Harbor Symp. Quant. Biol. 39, 637.

          Citations

          Title
        • Ricardo Sanchez-Prieto, et al. (1999) An Association Between Viral Genes and Human Oncogenic Alterations: The Adenovirus E1A Induces the Ewing Tumor Fusion Transcript EWS-FLI1. Nature Medicine 5, 1076-1079.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision30-January-2009 JSW
          ApplicationImmunofluorescence (2-5 µg/ml, see application references)
          Immunoprecipitation (1-2 µg antibody/mg total protein)
          Immunoblotting (1 µg/ml; see application references)
          Immunocytochemistry (see application references)
          Frozen Sections (2-4 µg/ml)
          Paraffin Sections (2-4 µg/ml; heat pre-treatment required; see application references)
          DescriptionProtein A purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes adenovirus-infected cells and tissue.
          BackgroundThe early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the leftmost 11% of the viral genome and consists of two transcriptional units, E1A and E1B. Region E1A is sufficient for partial transformation and immortalization of primary cells, whereas the E1B function is normally required for complete transformation. In addition to their essential role in transformation, E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection, and are able to either activate or repress the transcription of specific cellular genes. E1A oncogene proteins form specific complexes with cellular proteins including the anti-oncogene retinoblastoma susceptibility gene product, p105. The consequence of this interaction is inhibition of the cell cycle arresting function of p105.
          HostMouse
          Immunogenadenovirus 2 E1A protein
          CloneM73
          IsotypeIgG2a
          Speciesadenovirus infected cells
          Positive controlHEK293 cells or adenovirus-infected tissue
          Negative controlHS27 cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsImmunoprecipitates the adenovirus 2 and 5 E1A proteins (3 bands at ~30-50 kDa) as well as associated proteins. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
          Toxicity Standard Handling
          ReferencesWhyte, P., et al. 1998. Nature 334, 124.
          Ziff, E. et al. 1985. Cell 40, 705.
          Houweling, A., et al. 1980. Virology 105, 537.
          Berk, A.J., et al. 1979. Cell 17, 935.
          Jones, N. and Shenk, T. 1979. Proc. Natl. Acad. Sci. USA 76, 3665.
          Shiroki, K., et al. 1979. Virology 95, 127.
          Gallimore, P.H., et al. 1974. J. Mol. Biol. 89, 49.
          Graham, F.L., et al. 1974. Cold Spring Harbor Symp. Quant. Biol. 39, 637.
          Citation
        • Ricardo Sanchez-Prieto, et al. (1999) An Association Between Viral Genes and Human Oncogenic Alterations: The Adenovirus E1A Induces the Ewing Tumor Fusion Transcript EWS-FLI1. Nature Medicine 5, 1076-1079.
        • Application referencesImmunoblotting Stiewe T., et al. 2000. Cancer Res. 60, 3957. Nishihara, A., et al. 1999. J. Biol. Chem. 274, 28716. Sanchez-Prieto, R., et al. 1999. Nat. Med. 5, 1076. van den Berg, A., et al. 2005. Respir. Res. 6, 111. Immunocytochemistry van den Berg, A., et al. 2005. Respir. Res. 6, 111. Immunofluoresecence Harlow, E., et al. 1986. Mol. Cell. Biol. 6, 1579. Harlow, E., et al. 1985. J. Virol. 3, 533. Original Clone Harlow, E., et al. 1985. J. Virol. 3, 533. Paraffin Sections Nemunaitis, H., et al. 2000 Cancer Res. 60, 6359.