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05-717 | Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2

05-717
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Rb, Ca FC, IHC, WB M Purified Monoclonal Antibody
      Description
      Catalogue Number05-717
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2
      Alternate Names
      • adenosine A2 receptor
      • adenosine A2a receptor
      • adenosine receptor subtype A2a
      Background InformationAdenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationPurified mouse monoclonal IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Applications
      ApplicationAnti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2 is an antibody against Adenosine Receptor A2a clone 7F6-G5-A2 for use in FC, IH & WB.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry: 1 μg/mL of a previous lot detected Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice.

      FACS Analysis: A previous lot of this antibody has been reported by an independent laboratory to detect Adenosine Receptor A2A in transfected HEK293 cells (Koshiba, M., 1999).
      Biological Information
      Immunogen6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. Clone 7F6-G5-A2.
      Clone7F6-G5-A2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes adenosine receptor A2A, Mr 43 kDa.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Rabbit
      • Canine
      Species Reactivity NoteHuman, mouse, rat, rabbit, and dog. Cross-reactivity with guinea pig also reported.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
      Gene Symbol
      • ADORA2A
      • ADORA2
      • RDC8
      • hA2aR
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29274 # Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
      SIZE: 412 amino acids; 44707 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P29274 ## Belongs to the G-protein coupled receptor 1 family.
      Molecular Weight~43 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunohistochemistry.

      Western Blot Analysis:
      A 1:1000–1:5000 dilution of this lot of antibody detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras (Rosin, D.L., 1998).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Adenosine Receptor A2A (mouse monoclonal IgG2a, clone 7F6-G5-A2) - DAM1418763 DAM1418763
      Anti-Adenosine Receptor A2a clone 7F6-G5-A2 - DAM1588213 DAM1588213
      Anti-Adenosine Receptor A2a, -2571183 2571183
      Anti-Adenosine Receptor A2a, -2584409 2584409
      Anti-Adenosine Receptor A2a, -2607258 2607258
      Anti-Adenosine Receptor A2a, -2691210 2691210
      Anti-Adenosine Receptor A2a, -2700800 2700800
      Anti-Adenosine Receptor A2a, -2726718 2726718
      Anti-Adenosine Receptor A2a, -2762288 2762288
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 2475729
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - 2032289 2032289
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - 2048879 2048879
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - 2064513 2064513
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - 2189325 2189325
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - 2204914 2204914
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - 2287195 2287195
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - 2361842 2361842
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - 2495652 2495652
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - DAM1713175 DAM1713175
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - DAM1764399 DAM1764399
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - DAM1780362 DAM1780362
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 - JBC1858526 JBC1858526
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 -2502957 2502957
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2 -2716682 2716682
      Anti-Adenosine Receptor A2a, clone 7F6-G5-A2_2787693 2787693
      Anti-Adenosine Receptor A2a,clone 7F6-G5-A2 -2660596 2660596
      Anti-Adenosine Receptor A<sub>2A</sub>, clone 7F6-G5-A2 - 24519 24519
      Anti-Adenosine Receptor A<sub>2A</sub>, clone 7F6-G5-A2 - 28777 28777
      Anti-Adenosine Receptor A<sub>2A</sub>, clone 7F6-G5-A2 - DAM1408806 DAM1408806
      Anti-Adenosine Receptor A<sub>2A</sub>, clone 7F6-G5-A2 - JBC1362018 JBC1362018

      References | 12 Available | See All References

      Reference overviewApplicationPub Med ID
      Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory.
      Orr, AG; Hsiao, EC; Wang, MM; Ho, K; Kim, DH; Wang, X; Guo, W; Kang, J; Yu, GQ; Adame, A; Devidze, N; Dubal, DB; Masliah, E; Conklin, BR; Mucke, L
      Nature neuroscience 18 423-34 2015

      Show Abstract
      25622143 25622143
      Deficits in endogenous adenosine formation by ecto-5'-nucleotidase/CD73 impair neuromuscular transmission and immune competence in experimental autoimmune myasthenia gravis.
      Oliveira, L; Correia, A; Cristina Costa, A; Guerra-Gomes, S; Ferreirinha, F; Magalhães-Cardoso, MT; Vilanova, M; Correia-de-Sá, P
      Mediators of inflammation 2015 460610 2015

      Show Abstract
      Immunohistochemistry25691808 25691808
      Adenosine A(2A) receptor up-regulates retinal wave frequency via starburst amacrine cells in the developing rat retina.
      Huang, PC; Hsiao, YT; Kao, SY; Chen, CF; Chen, YC; Chiang, CW; Lee, CF; Lu, JC; Chern, Y; Wang, CT
      PloS one 9 e95090 2014

      Show Abstract
      24777042 24777042
      Overexpression of Adenosine A2A Receptors in Rats: Effects on Depression, Locomotion, and Anxiety.
      Coelho, JE; Alves, P; Canas, PM; Valadas, JS; Shmidt, T; Batalha, VL; Ferreira, DG; Ribeiro, JA; Bader, M; Cunha, RA; do Couto, FS; Lopes, LV
      Frontiers in psychiatry 5 67 2014

      Show Abstract
      24982640 24982640
      Adenosine A2A receptors modulate glutamate uptake in cultured astrocytes and gliosomes.
      Matos, Marco, et al.
      Glia, 60: 702-16 (2012) 2012

      Show Abstract
      22298379 22298379
      Adenosine A2A receptor in the monkey basal ganglia: Ultrastructural localization and colocalization with the metabotropic glutamate receptor 5 in the striatum.
      James W Bogenpohl,Stefanie L Ritter,Randy A Hall,Yoland Smith
      The Journal of comparative neurology 520 2012

      Show Abstract
      21858817 21858817
      Detection of antigen interactions ex vivo by proximity ligation assay: endogenous dopamine D2-adenosine A2A receptor complexes in the striatum.
      Trifilieff, P; Rives, ML; Urizar, E; Piskorowski, RA; Vishwasrao, HD; Castrillon, J; Schmauss, C; Slättman, M; Gullberg, M; Javitch, JA
      BioTechniques 51 111-8 2011

      Show Abstract
      21806555 21806555
      Striatal adenosine A2A and cannabinoid CB1 receptors form functional heteromeric complexes that mediate the motor effects of cannabinoids.
      Carriba, P; Ortiz, O; Patkar, K; Justinova, Z; Stroik, J; Themann, A; Müller, C; Woods, AS; Hope, BT; Ciruela, F; Casadó, V; Canela, EI; Lluis, C; Goldberg, SR; Moratalla, R; Franco, R; Ferré, S
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 32 2249-59 2007

      Show Abstract
      17356572 17356572
      Forebrain adenosine A2A receptors contribute to L-3,4-dihydroxyphenylalanine-induced dyskinesia in hemiparkinsonian mice.
      Xiao, D; Bastia, E; Xu, YH; Benn, CL; Cha, JH; Peterson, TS; Chen, JF; Schwarzschild, MA
      The Journal of neuroscience : the official journal of the Society for Neuroscience 26 13548-55 2006

      Show Abstract
      17192438 17192438
      Ultrastructural localization of adenosine A2A receptors suggests multiple cellular sites for modulation of GABAergic neurons in rat striatum.
      Hettinger, B D, et al.
      J. Comp. Neurol., 431: 331-46 (2001) 2001

      Show Abstract
      Immunocytochemistry11170009 11170009

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies