A part of MilliporeSigma

AB5694 | Anti-ATH 5 Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IHC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB5694
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-ATH 5 Antibody
      Alternate Names
      • MATH5
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      ApplicationDetect ATH 5 using this Anti-ATH 5 Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:200-1:1,000 using ECL.

      Immunohistochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from mouse ATH 5.
      SpecificityRecognizes ATH 5 (MATH5) (NeuroD family), a Helix-loop-helix class of transcription factor.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryATOH7 is a member of the family of basic helix-loop-helix (bHLH) proteins with similarity to Drosophila 'atonal,' a proneural bHLH gene that controls photoreceptor development (Brown et al., 2002 [PubMed 11889557]).[supplied by OMIM]
      Gene Symbol
      • ATOH7
      • Math5
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8N100 # Transcription factor involved in the differentiation of retinal ganglion cells (By similarity).
      SIZE: 152 amino acids; 16871 Da
      SUBCELLULAR LOCATION: Nucleus (Probable).
      SIMILARITY: SwissProt: Q8N100 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.


      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-ATH 5 (MATH5) 2459030
      RABBIT ANTI-ATH 5 (MATH5) -2560994 2560994
      RABBIT ANTI-ATH 5 (MATH5) -2634650 2634650
      RABBIT ANTI-ATH 5 (MATH5) -2650880 2650880
      RABBIT ANTI-ATH 5 (MATH5) -2665010 2665010
      RABBIT ANTI-ATH 5 (MATH5) -2689078 2689078
      RABBIT ANTI-ATH 5 (MATH5) -2728431 2728431
      RABBIT ANTI-ATH 5 (MATH5) -2766505 2766505
      RABBIT ANTI-ATH 5 (MATH5) AFFINITY PURIFIED - 2712412 2712412

      References | 11 Available | See All References

      Reference overviewApplicationPub Med ID
      Adult ciliary epithelial stem cells generate functional neurons and differentiate into both early and late born retinal neurons under non-cell autonomous influences.
      Del Debbio, CB; Peng, X; Xiong, H; Ahmad, I
      BMC neuroscience 14 130 2013

      Show Abstract
      24148749 24148749
      Cathepsin B overexpression due to acid sphingomyelinase ablation promotes liver fibrosis in Niemann-Pick disease.
      Moles, A; Tarrats, N; Fernández-Checa, JC; Marí, M
      The Journal of biological chemistry 287 1178-88 2012

      Show Abstract
      22102288 22102288
      Caspase-1-mediated regulation of fibrogenesis in diet-induced steatohepatitis.
      Dixon, LJ; Berk, M; Thapaliya, S; Papouchado, BG; Feldstein, AE
      Laboratory investigation; a journal of technical methods and pathology 92 713-23 2012

      Show Abstract
      Immunohistochemistry22411067 22411067
      Ingredients of Huangqi decoction slow biliary fibrosis progression by inhibiting the activation of the transforming growth factor-beta signaling pathway.
      Jin-Xing Du,Ming-Yu Sun,Guang-Li Du,Feng-Hua Li,Cheng Liu,Yong-Ping Mu,Gao-Feng Chen,Ai-Hua Long,Yan-Qin Bian,Jia Liu,Cheng-Hai Liu,Yi-Yang Hu,Lie-Ming Xu,Ping Liu
      BMC complementary and alternative medicine 12 2012

      Show Abstract
      22471627 22471627
      Regeneration of uterine horns in rats by collagen scaffolds loaded with collagen-binding human basic fibroblast growth factor.
      Li X, Sun H, Lin N, Hou X, Wang J, Zhou B, Xu P, Xiao Z, Chen B, Dai J, Hu Y.
      Biomaterials 32 8172-81 2011

      Show Abstract
      21821282 21821282
      Prostate epithelial Pten/TP53 loss leads to transformation of multipotential progenitors and epithelial to mesenchymal transition.
      Martin, P; Liu, YN; Pierce, R; Abou-Kheir, W; Casey, O; Seng, V; Camacho, D; Simpson, RM; Kelly, K
      The American journal of pathology 179 422-35 2011

      Show Abstract
      Western Blotting21703421 21703421
      Angiopoietin-1 is essential in mouse vasculature during development and in response to injury.
      Jeansson, M; Gawlik, A; Anderson, G; Li, C; Kerjaschki, D; Henkelman, M; Quaggin, SE
      The Journal of clinical investigation 121 2278-89 2011

      Show Abstract Full Text Article
      21606590 21606590
      Dual origin of mesenchymal stem cells contributing to organ growth and repair.
      Feng, J; Mantesso, A; De Bari, C; Nishiyama, A; Sharpe, PT
      Proceedings of the National Academy of Sciences of the United States of America 108 6503-8 2011

      Show Abstract
      21464310 21464310
      tPA is a potent mitogen for renal interstitial fibroblasts: role of beta1 integrin/focal adhesion kinase signaling.
      Hao, S; Shen, H; Hou, Y; Mars, WM; Liu, Y
      The American journal of pathology 177 1164-75 2010

      Show Abstract
      20639453 20639453
      A critical analysis of Atoh7 (Math5) mRNA splicing in the developing mouse retina.
      Prasov, L; Brown, NL; Glaser, T
      PloS one 5 e12315 2010

      Show Abstract
      20808762 20808762

      Data Sheet

      Anti-ATH 5 - Data Sheet