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ABS54 | Anti-AS160 Antibody

ABS54
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Ca, R WB, IP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABS54
      DescriptionAnti-AS160 Antibody
      Alternate Names
      • Acrg embryonic lethality minimal region ortholog
      • Akt substrate of 160 kDa
      • TBC (Tre-2, BUB2, CDC16) domain-containing protein
      • TBC1 domain family, member
      Background InformationAS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEPG2 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-AS160 Antibody detects level of AS160 & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected AS160 on 10 µg of HEPG2 cell lysate.

      Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated AS160 from HEPG2 lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of mouse AS160.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes AS160 at the C-terminus.
      Species Reactivity
      • Mouse
      • Human
      • Canine
      • Rat
      Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react on canine and rat based on 100% sequence homology.
      Chimpanzee, equine, and ox (90% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AS160
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake (By similarity).

      SUBCELLULAR LOCATION: Cytoplasm. Note: Cytoplasmic perinuclear (by similarity).

      TISSUE SPECIFICTY: Widely expressed, including in pancreatic beta cells.

      PTM: Phosphorylated by AKT1; insulin-induced.

      Insulin-stimulated phosphorylation is required for GLUT4 translocation. Has no effect on GLUT4 internalization.

      SEQUENCE SIMILARITIES: Contains 2 PID domains.

      Contains 1 Rab-GAP TBC domain.
      Molecular Weight~ 160 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEPG2 cell lysate.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected AS160 on 10 µg of HEPG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-AS160 - 2128263 2128263
      Anti-AS160 - 2430500 2430500
      Anti-AS160 - 1975955 1975955
      Anti-AS160 - 2006972 2006972
      Anti-AS160 - 2219376 2219376
      Anti-AS160 - NG1820259 NG1820259
      Anti-AS160 - NG1940974 NG1940974
      Anti-AS160 - NRG1732256 NRG1732256
      Anti-AS160 -2499674 2499674
      Anti-AS160 -2551192 2551192
      Anti-AS160 -2572970 2572970
      Anti-AS160 -2585826 2585826
      Anti-AS160 -2736603 2736603

      References

      Reference overviewApplicationPub Med ID
      Lipid mixtures containing a very high proportion of saturated fatty acids only modestly impair insulin signaling in cultured muscle cells.
      Newsom, SA; Everett, AC; Park, S; Van Pelt, DW; Hinko, A; Horowitz, JF
      PloS one 10 e0120871 2015

      Show Abstract
      Western Blotting25793412 25793412
      Proteomic analysis of protein palmitoylation in adipocytes.
      Ren, W; Jhala, US; Du, K
      Adipocyte 2 17-28 2013

      Show Abstract
      23599907 23599907
      Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in skeletal muscle without sustained increases in kinase phosphorylation.
      Schweitzer, GG; Arias, EB; Cartee, GD
      Journal of applied physiology (Bethesda, Md. : 1985) 113 1852-61 2012

      Show Abstract
      22936728 22936728
      Activity profiles of cholinergic and intermingled GABAergic and putative glutamatergic neurons in the pontomesencephalic tegmentum of urethane-anesthetized rats.
      Soufiane Boucetta,Barbara E Jones
      The Journal of neuroscience : the official journal of the Society for Neuroscience 29 2009

      Show Abstract
      19357291 19357291