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MABN380 | Anti-APP-C99 Antibody, clone mC99(70–80)

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IP, IHC, ICC M Ascites Monoclonal Antibody
      Catalogue NumberMABN380
      DescriptionAnti-APP-C99 Antibody, clone mC99(70–80)
      Alternate Names
      • Amyloid beta A4 protein
      • ABPP
      • APPI
      • APP
      • Alzheimer disease amyloid protein
      • Cerebral vascular amyloid peptide
      • CVAP
      • PreA4
      • Protease nexin-II
      • PN-II
      Background InformationAmyloid Precursor Protein (APP) is expressed in the brain, kidney, heart, and spleen of fetal tissues; it is induced during neuronal differentiation. The most-substantiated role for APP is in synaptic formation and repair; its expression is upregulated during neuronal differentiation and after neural injury. In adult brain, highest expression of APP is found in the frontal lobe of the cortex. Moderate expression is observed in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Defects in APP are a cause of autosomal dominant Alzheimer's disease (AD). UP on intracerebroventricular injection of this antibody, A-beta levels significantly decreased in the brain of APPPS1 mouse model of AD. A related antibody, Cat. No. MABN381, Anti-APP-C99, clone mC99(1-7) was raised against a slightly different region of the same APP-C99 protein.
      Product Information
      PresentationMouse monoclonal IgGκ in ascites containing 0.05% sodium azide.
      ApplicationAnti-APP-C99 Antibody, clone mC99(70–80) is a highly specific mouse monoclonal antibody, that targets APP & has been tested in western blotting, IP, IHC & ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated APP-C99 in HEK APPSwe cell lysate (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

      Immunohistochemistry Analysis: A representative lot detected APP-C99 in APPS1 mice and in human Alzheimer brain tissues
      (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).

      Immunofluoresence Analysis: A representative lot detected APP-C99 in HEK APPSwe cells (Houacine, J., et al. (2012). Neurobiol Aging. 33(11):2704-2714.).
      Biological Information
      ImmunogenRecombinant protein corresponding to Human APP-C99.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~95 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in DAPT treated HEK293 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected APP-C99 in 10 µg of DAPT treated HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-APP-C99, clone mC99(70#8211;80) - Q2226883 Q2226883
      Anti-APP-C99, clone mC99(70–80) -2738975 2738975
      Anti-APP-C99, clone mC99(70–80) -2759815 2759815
      Anti-APP-C99, clone mC99(70–80) -2785783 2785783
      Anti-APP-C99, clone mC99(70–80) -2823183 2823183


      Reference overviewPub Med ID
      Selective neutralization of APP-C99 with monoclonal antibodies reduces the production of Alzheimer's Aβ peptides.
      Houacine, Jemila, et al.
      Neurobiol. Aging, 33: 2704-14 (2012) 2012

      Show Abstract
      22317957 22317957

      Newsletters / Publications

      Research Focus - Volume 2, 2013