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ABC1696 | Anti-APH-1A Antibody

100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R WB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABC1696
      DescriptionAnti-APH-1A Antibody
      Alternate Names
      • Gamma-secretase subunit APH-1A
      • Anterior pharynx defective 1 homolog A
      • APH-1a
      • Aph-1alpha
      • Presenilin-stabilization factor
      Background InformationGamma-secretase subunit APH-1A (UniProt Q96BI3; also known as Anterior pharynx defective 1 homolog A, APH-1a, Aph-1alpha, Presenilin-stabilization factor) is encoded by the APH1A (also known as PSF, CGI-78) gene (ORF UNQ579/PRO1141; Gene ID 51107) in human. Gamma-secretase catalyzes the final cleavage of beta-amyloid precursor protein to generate amyloid-beta peptide, the principal component of amyloid plaques found in Alzheimer's diseased (AD) brains. Gamma-secretase is a complex composed of four integral membrane proteins, presenilin (PS), nicastrin (NCT), PEN-2, and APH-1. The PS component could be either a PS1 or PS2 and the APH component could be either an APH-1a or APH-1b, alternative splicings of these four proteins contribute to further heterogeneity among gamma-secretase complexes. The gamma-secretase complex is assembled in the ER, where NCT and APH-1 form an initial scaffold for the PS holoprotein, which becomes stabilized upon interaction with the NCT/APH-1. Subsequent addition of PEN-2 to this trimeric assembly intermediate triggers the endoproteolytic cleavage of the PS holoprotein. The fourth transmembrane domain (TMD4) of APH-1, the PEN-2 C-terminal end, the NCT TMD domain, and the PS1 C-terminal end are shown to be essential for functional gamma-secretase complex assembly. APH-1a is a seven-transmembrane (a.a. 3-23, 32-52, 69-89, 119-139, 159-179, 187-207, 214-234) protein with a lumenal N-terminus and a cytoplasmic C-terminal tail (a.a. 235-265).
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-APH-1A Antibody is an antibody against APH-1A for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence from the C-terminal cytoplasmic tail of human APH-1A.
      EpitopeC-terminal cytoplasmic tail.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityExpected to react with all three spliced isoforms of human APH-1A reported by UniProt (Q96BI3).
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat. Predicted to react with Mouse, Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APH1A
      • PSF
      • CGI-78
      • UNQ579/PRO1141
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~32 kDa observed. 29.00 kDa (isoform 1; isoform L; isoform Alph-alpha1), 26.84 kDa (isoform 2; isoform S; isoform Alph-alpha2), 20.98 kDa (isoform 3) calculated.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected APH-1A in 10 µg of human kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies