MABS1257 Anti-APC4 Antibody, clone CIV1.1

MABS1257
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IH(P), IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS1257
      DescriptionAnti-APC4 Antibody, clone CIV1.1
      Alternate Names
      • Anaphase-promoting complex subunit 4
      • APC4
      • Cyclosome subunit 4
      Background InformationAnaphase-promoting complex subunit 4 (UniProt Q9UJX5; also known as APC4, Cyclosome subunit 4) is encoded by the ANAPC4 (also known as APC4) gene (Gene ID 29945) in human. APC4 is a component of the anaphase-promoting complex or cyclosome (APC/C). APC/C is an E3 ubiquitin ligase complex that promotes the transition from metaphase to anaphase of the cell cycle by targeting specific proteins for proteasomal degradation at specific times under the control of the spindle assembly checkpoint (SAC). The catalytic core of the APC/C consists of the cullin subunit APC2 and RING H2 domain subunit APC11. Other APC/C components, including APC4, are composed multiple repeat motifs with the main purpose of providing molecular scaffold support. APC4 contains WD repeats and has a molecular weight of approximately 92 kDa. APC4 is located in the nucleus during interphase and at the centrosome during metaphase/anaphase.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
      Applications
      ApplicationAnti-APC4, clone CIV1.1 Antibody, Cat. No. MABS1257, is a highly specific mouse monoclonal antibody that targets APC4 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC4 in human spleen tissue section.

      Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Cdc20, Mad2, and BubR1 with APC4 from prometaphase HeLa cells (Di Fiore, B., et al. (2015). Dev. Cell. 32(3):358-372).

      Western Blotting Analysis: A representative lot detected APC4 in whole cell lysates, as well as in FLAG-tagged cyclin A and FLAG-tagged BubR1 immunoprecipitates from prometaphase HeLa cells (Di Fiore, B., et al. (2015). Dev. Cell. 32(3):358-372).
      Biological Information
      ImmunogenLinear peptide corresponding to human APC4 C-terminal end sequence.
      EpitopeC-terminus
      CloneCIV1.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone CIV1.1 target human APC4 C-terminal end sequence present in spliced isoforms 1 and 3, but absent in isoform 2 reported by UniProt (Q9UJX5).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ANAPC4
      • APC4
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight92.12/92.22 kDa (isoform 1/3) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human uterus tissue section.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected APC4 in human uterus tissue section.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications