A part of MilliporeSigma

MABS1228 | Anti-ANKRD1/CARP Antibody, clone 12G8.2

100 μg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R WB, IH(P) M Purified Monoclonal Antibody
      Catalogue NumberMABS1228
      DescriptionAnti-ANKRD1/CARP Antibody, clone 12G8.2
      Alternate Names
      • Ankyrin repeat domain-containing protein 1
      • Cardiac ankyrin repeat protein
      • Cytokine-inducible gene C-193 protein
      • Cytokine-inducible nuclear protein
      Background InformationAnkyrin repeat domain-containing protein 1 (UniProt Q15327; also known as Cardiac ankyrin repeat protein, Cytokine-inducible gene C-193 protein, Cytokine-inducible nuclear protein) is encoded by the ANKRD1 (also known as C193, CARP, HA1A2) gene (Gene ID 27063) in human. ANKRD1/CARP belongs to a family of conserved muscle ankyrin repeat proteins (MARPs), members of which also include ANKRD2 and diabetes ankyrin repeat protein (DARP, ANKRD23). ANKRD1 is highly expressed in cardiomyocytes and known to interact with myopalladin and titin, implicating its roles in myopalladin complex-dependent sarcomere integrity maintenance and stress-induced gene regulations medidated by the titin complex. ANKRD1 is up-regulated in patients with dilated cardiomyopathy and in animal models of cardiac hypertrophy induced by pressure overload and adrenergic stimulation. ANKRD1 is shown to regulate the hypertrophic agonist phenylephrine-/PE-induced α-adrenergic signaling pathway in neonatal rat ventricular myocytes, where it recruits and localizes ERK1/2 and GATA4 in a sarcomeric complex, resulting in enhanced GATA4 phosphorylation and subsequent ANKRD1 complex nuclear translocation and gene activation.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-ANKRD1/CARP Antibody, clone 12G8.2 is an antibody against ANKRD1/CARP for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected ANKRD1/CARP in human and rat heart tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human ANKRD1/CARP fragment corresponding ankyrin repeats 4&5.
      EpitopeAnkyrin repeats 4&5.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ANKRD1
      • C193
      • CARP
      • HA1A2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~37 kDa observed. 36.25 kDa calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human heart tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected ANKRD1/CARP in 10 µg of human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information