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PC287 | Anti-AML3 Rabbit pAb

PC287
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      PC287-100UG
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      Stocked 
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~65 kDa, the ~60 kDa, and the ~45 kDa AML3 proteins in transfected COS or BRL (buffalo rat liver) cells.
          Catalogue NumberPC287
          Brand Family Calbiochem®
          References
          ReferencesBanerjee, C., et al. 1997. J. Cell Biochem. 66, 1.
          Lo Coco, F., et al. 1997. Haematologica 82, 364.
          Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
          Levanon, D., et al. 1994. Genomics 23, 425.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Positive controlTransfected COS or buffalo rat liver (BRL) cells
          PreservativeNone
          Applications
          Application ReferencesGel Shift Banerjee, C., et al. 1997. J. Cell Biochem. 66, 1. Lo Coco, F., et al. 1997. Haematologica 82, 364. Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251. Levanon, D., et al. 1994. Genomics 23, 425.
          Key Applications Gel Shift
          Immunoblotting (Western Blotting)
          Not Frozen Sections
          Not Immunofluorescence
          Not Immunoprecipitation
          Not Paraffin Sections
          Application NotesGel Shift (see application references)
          Immunoblotting (2.5 µg/ml)
          Frozen Sections (not recommended)
          Immunofluorescence (not recommended)
          Immunoprecipitation (not recommended)
          Paraffin Sections (not recommended)
          Application CommentsThe ~45 kDa species is not detected in mature osteoblasts. For gel shift assay resuspend in 100 µl of buffer. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide [(K)TDVPRRISDDDTATSD] corresponding to amino acids 319-334 from human AML3
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          PC287

          References

          Reference overview
          Banerjee, C., et al. 1997. J. Cell Biochem. 66, 1.
          Lo Coco, F., et al. 1997. Haematologica 82, 364.
          Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
          Levanon, D., et al. 1994. Genomics 23, 425.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-July-2009 RJH
          ApplicationGel Shift (see application references)
          Immunoblotting (2.5 µg/ml)
          Frozen Sections (not recommended)
          Immunofluorescence (not recommended)
          Immunoprecipitation (not recommended)
          Paraffin Sections (not recommended)
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes proteins of ~65, ~60 and ~45 kDa in osteoclasts and bone.
          BackgroundThe family of nuclear transcription factors represented by AML are important transactivators of tissue-specific genes of hematopoietic and bone cell lineage. There are currently three mammalian proteins that are members of this family, AML1, AML2, and AML3. The AML proteins in turn form heterodimers with another protein, CBFβ and it is this complex that produces the transcription factor activity. Within this complex the AML protein provides the DNA binding function. The AML gene was discovered in 1991 by cloning the t(8;21) translocation that is common to AML. Structurally the AML protein contains a transactivation domain at the C-terminus is homologous with the Drosophila runt gene that acts as the DNA binding area of the protein. AML3, the third member of the AML family, is unique from the other family members in that it also possesses osteoblast-specific transcriptional regulation properties. AML3 has been demonstrated as a single transcript of 5.4 kb in bone tissues where the protein encoded by the gene has been shown to be present in the osteoblast-specific promoter binding complex. Like other AML proteins, AML3 contains the runt domain, a putative ATP binding site, and a proline and serine rich C-terminal half. AML3 is also the predominant runt domain containing transcription factor in Buffalo Rat liver cells.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide [(K)TDVPRRISDDDTATSD] corresponding to amino acids 319-334 from human AML3
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlTransfected COS or buffalo rat liver (BRL) cells
          FormLiquid
          FormulationIn PBS.
          PreservativeNone
          CommentsThe ~45 kDa species is not detected in mature osteoblasts. For gel shift assay resuspend in 100 µl of buffer. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°).
          Toxicity Standard Handling
          ReferencesBanerjee, C., et al. 1997. J. Cell Biochem. 66, 1.
          Lo Coco, F., et al. 1997. Haematologica 82, 364.
          Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
          Levanon, D., et al. 1994. Genomics 23, 425.
          Application referencesGel Shift Banerjee, C., et al. 1997. J. Cell Biochem. 66, 1. Lo Coco, F., et al. 1997. Haematologica 82, 364. Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251. Levanon, D., et al. 1994. Genomics 23, 425.