ST1507 Anti-AKR1B10 Mouse mAb (1A6)

ST1507
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1507-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.
          Catalogue NumberST1507
          Brand Family Calbiochem®
          SynonymsAnti-Aldo-Keto Reductase 1B10
          Application Data
          Detection of human AKR1B10 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human AKR1B10 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (3 µg/ml). Detection: colorimetric (DAB).

          Detection of human AKR1B10 by immunocytochemistry. Sample: HeLa cellsfixed in Paraformaldehyde. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (10 µg/ml). Detection: fluorescence.
          References
          ReferencesAhn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
          Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.
          Product Information
          Formliquid
          FormulationIn PBS, pH 7.2.
          Positive controlHepG2 cells, Human Colon tissue, HeLa cells
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
          ImmunogenNA
          Clone1A6
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-AKR1B10 Mouse mAb (1A6) SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Ahn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
          Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-November-2010 RFH
          SynonymsAnti-Aldo-Keto Reductase 1B10
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human AKR1B10 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human AKR1B10 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (3 µg/ml). Detection: colorimetric (DAB).

          Detection of human AKR1B10 by immunocytochemistry. Sample: HeLa cellsfixed in Paraformaldehyde. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (10 µg/ml). Detection: fluorescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa AKR1B10 protein.
          BackgroundThis gene product encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis.
          HostMouse
          Immunogen speciesNA
          ImmunogenA recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
          Clone1A6
          IsotypeIgG2a
          Specieshuman
          Positive controlHepG2 cells, Human Colon tissue, HeLa cells
          Formliquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review
          ReferencesAhn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
          Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.